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When a Tree Falls in the Woods: The Gravitropic Response in Poplar

Nancy R. Hofmann
Nancy R. Hofmann
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Published October 2015. DOI: https://doi.org/10.1105/tpc.15.00824

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If a tree survives a fall in the woods, the main stem grows upward from its new position. In angiosperm trees, this growth reorientation is achieved via differential activity in the cambium, which produces tension wood on the side of the stem now facing upward and opposite wood on the bottom side (Ruelle, 2014). Whereas opposite wood is phenotypically similar to “normal” wood formed by upright stems, tension wood contains characteristic tension wood fibers with an additional, tertiary cell wall, the gelatinous layer (G-layer). The G-layer has been proposed to be responsible for the tensile force that causes the stem to bend upright as it grows (Mellerowicz and Gorshkova, 2012). Tension wood development likely involves hormones including auxin, ethylene, and gibberellic acid, although their precise roles have been difficult to ascertain. Cell wall- and hormone-related genes are differentially expressed in tension wood, but the underlying transcriptional regulation networks are not clear. New work from Gerttula et al. (2015) integrates experimental and computational data to describe the genetic and molecular processes underlying these fascinating developmental responses.

The poplar (Populus spp) transcription factor ARBORKNOX2 (ARK2) is expressed in the cambium and affects wood development (Du et al., 2009). Here, Gerttula et al. established that ARK2 is important in the gravibending response. When placed on their sides, poplar saplings with reduced ARK2 levels (miRNA-ARK2) bent upward later and to a lesser degree compared with the wild type, whereas those overexpressing ARK2 (OE-ARK2) showed greater gravibending (see figure). Interestingly, the degree of gravibending was not associated with the number of tension wood fibers formed, but instead with their rate of maturation, particularly of the G-layer. Thus, increased ARK2 expression confers earlier maturation of tension wood fibers, which leads to greater bending.

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Gravibending response in poplar. Two weeks after being placed on their sides, poplar downregulated in ARK2 (A) show weaker gravibending than wild-type (B) and ARK2-overexpressing (C) saplings. Bars = 10 cm. (Reprinted from Gerttula et al. [2015], Figure 1.)

Gerttula et al. found that the PINFORMED3 (PIN3) auxin transporter was uniformly distributed in the plasma membrane of endodermal cells in the inner cortex of young upright stems, as well as in the secondary phloem in older stems. These same cells contained amyloplasts, suggesting that they could act as gravisensors. When the trees were turned on their sides, PIN3 relocated to the ground-facing side of the cells. Importantly, this PIN3 localization would lead to auxin movement toward the cambium on the tension wood side and away from the cambium on the opposite wood side, which could trigger the differential cambium activity that leads to opposite and tension wood production.

The authors analyzed transcriptome data from normal wood, tension wood, and opposite wood, the latter of which has typically been thought a passive participant in the gravitropic responses. Their comprehensive gene regulatory network analysis placed genes into modules correlated with tension wood traits that could be further dissected to identify putative regulators of different aspects of graviresponse and wood formation.

This work from Gerttula et al. beautifully applies a range of molecular techniques to poplar to address several key questions in wood formation and gravitropic responses in trees. The results suggest that endodermis and secondary phloem cells serve as the gravisensors and support a simple model in which this sensing leads to lateral auxin transport that underlies different responses to gravity in different sides of the stem. Furthermore, the network analysis provides candidate genes for future study and predictive models for hypothesis generation and testing in a long-lived forest tree species.

Footnotes

  • www.plantcell.org/cgi/doi/10.1105/tpc.15.00824

References

  1. ↵
    1. Du, J.,
    2. Mansfield, S.D.,
    3. Groover, A.T.
    (2009). The Populus homeobox gene ARBORKNOX2 regulates cell differentiation during secondary growth. Plant J. 60: 1000–1014.
    OpenUrlCrossRefPubMed
  2. ↵
    1. Gerttula, S.,
    2. Zinkgraf, M.,
    3. Muday, G.,
    4. Lewis, D.,
    5. Ibatullin, F.M.,
    6. Brumer, H.,
    7. Hart, F.,
    8. Mansfield, S.D.,
    9. Filkov, V.,
    10. Groover, A.
    (2015). Transcriptional and hormonal regulation of gravitropism of woody stems in Populus. Plant Cell 27: 2800–2813.
    OpenUrlAbstract/FREE Full Text
  3. ↵
    1. Mellerowicz, E.J.,
    2. Gorshkova, T.A.
    (2012). Tensional stress generation in gelatinous fibres: a review and possible mechanism based on cell-wall structure and composition. J. Exp. Bot. 63: 551–565.
    OpenUrlAbstract/FREE Full Text
  4. ↵
    1. B. Gardiner,
    2. J. Barnett,
    3. P. Saranpää, and
    4. J. Gril
    1. Ruelle, J.
    (2014). Morphology, anatomy and ultrastructure of reaction wood. In The Biology of Reaction Wood, B. Gardiner, J. Barnett, P. Saranpää, and J. Gril, eds (Berlin, Heidelberg: Springer-Verlag), pp.13–35.
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When a Tree Falls in the Woods: The Gravitropic Response in Poplar
Nancy R. Hofmann
The Plant Cell Oct 2015, 27 (10) 2674; DOI: 10.1105/tpc.15.00824

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When a Tree Falls in the Woods: The Gravitropic Response in Poplar
Nancy R. Hofmann
The Plant Cell Oct 2015, 27 (10) 2674; DOI: 10.1105/tpc.15.00824
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The Plant Cell: 27 (10)
The Plant Cell
Vol. 27, Issue 10
Oct 2015
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