Skip to main content

Main menu

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Cell
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Cell

Advanced Search

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Follow PlantCell on Twitter
  • Visit PlantCell on Facebook
  • Visit Plantae
Research ArticleResearch Article
Open Access

KNOTTED1 Cofactors, BLH12 and BLH14, Regulate Internode Patterning and Vein Anastomosis in Maize

Katsutoshi Tsuda, Maria-Jazmin Abraham-Juarez, Akiteru Maeno, Zhaobin Dong, Dale Aromdee, Robert Meeley, Toshihiko Shiroishi, Ken-ichi Nonomura, Sarah Hake
Katsutoshi Tsuda
aExperimental Farm, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan
bDepartment of Genetics, School of Life Science, Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Katsutoshi Tsuda
Maria-Jazmin Abraham-Juarez
cPlant Gene Expression Center, U.S. Department of Agriculture-Agricultural Research Service, Plant and Microbial Biology Department, University of California at Berkeley, Albany, California 94710
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Maria-Jazmin Abraham-Juarez
Akiteru Maeno
dMammalian Genetics Laboratory, National Institute of Genetics, Mishima, Shizuoka, 411-8540, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhaobin Dong
cPlant Gene Expression Center, U.S. Department of Agriculture-Agricultural Research Service, Plant and Microbial Biology Department, University of California at Berkeley, Albany, California 94710
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dale Aromdee
cPlant Gene Expression Center, U.S. Department of Agriculture-Agricultural Research Service, Plant and Microbial Biology Department, University of California at Berkeley, Albany, California 94710
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Robert Meeley
ePioneer Hi-Bred International, Johnston, Iowa 50131
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Robert Meeley
Toshihiko Shiroishi
bDepartment of Genetics, School of Life Science, Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan
dMammalian Genetics Laboratory, National Institute of Genetics, Mishima, Shizuoka, 411-8540, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ken-ichi Nonomura
aExperimental Farm, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan
bDepartment of Genetics, School of Life Science, Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ken-ichi Nonomura
Sarah Hake
cPlant Gene Expression Center, U.S. Department of Agriculture-Agricultural Research Service, Plant and Microbial Biology Department, University of California at Berkeley, Albany, California 94710
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sarah Hake
  • For correspondence: hake@berkeley.edu

Published May 2017. DOI: https://doi.org/10.1105/tpc.16.00967

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading
  • © 2017 American Society of Plant Biologists. All rights reserved.

Abstract

Monocot stems lack the vascular cambium and instead have characteristic structures in which intercalary meristems generate internodes and veins remain separate and scattered. However, developmental processes of these unique structures have been poorly described. BELL1-like homeobox (BLH) transcription factors (TFs) are known to heterodimerize with KNOTTED1-like homeobox TFs to play crucial roles in shoot meristem maintenance, but their functions are elusive in monocots. We found that maize (Zea mays) BLH12 and BLH14 have redundant but important roles in stem development. BLH12/14 interact with KNOTTED1 (KN1) in vivo and accumulate in overlapping domains in shoot meristems, young stems, and provascular bundles. Similar to kn1 loss-of-function mutants, blh12 blh14 (blh12/14) double mutants fail to maintain axillary meristems. Unique to blh12/14 is an abnormal tassel branching and precocious internode differentiation that results in dwarfism and reduced veins in stems. Micro-computed tomography observation of vascular networks revealed that blh12/14 double mutants had reduced vein number due to fewer intermediate veins in leaves and precocious anastomosis in young stems. Based on these results, we propose two functions of BLH12/14 during stem development: (1) maintaining intercalary meristems that accumulate KN1 and prevent precocious internode differentiation and (2) preventing precocious anastomosis of provascular bundles in young stems to ensure the production of sufficient independent veins.

  • Glossary

    TF
    transcription factor
    BiFC
    bimolecular fluorescence complementation
    SAM
    shoot apical meristem
    CT
    computed tomography
    • Received December 28, 2016.
    • Revised March 3, 2017.
    • Accepted March 28, 2017.
    • Published April 5, 2017.

    View Full Text
    PreviousNext
    Back to top

    Table of Contents

    Print
    Download PDF
    Email Article

    Thank you for your interest in spreading the word on Plant Cell.

    NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

    Enter multiple addresses on separate lines or separate them with commas.
    KNOTTED1 Cofactors, BLH12 and BLH14, Regulate Internode Patterning and Vein Anastomosis in Maize
    (Your Name) has sent you a message from Plant Cell
    (Your Name) thought you would like to see the Plant Cell web site.
    CAPTCHA
    This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
    Citation Tools
    KNOTTED1 Cofactors, BLH12 and BLH14, Regulate Internode Patterning and Vein Anastomosis in Maize
    Katsutoshi Tsuda, Maria-Jazmin Abraham-Juarez, Akiteru Maeno, Zhaobin Dong, Dale Aromdee, Robert Meeley, Toshihiko Shiroishi, Ken-ichi Nonomura, Sarah Hake
    The Plant Cell May 2017, 29 (5) 1105-1118; DOI: 10.1105/tpc.16.00967

    Citation Manager Formats

    • BibTeX
    • Bookends
    • EasyBib
    • EndNote (tagged)
    • EndNote 8 (xml)
    • Medlars
    • Mendeley
    • Papers
    • RefWorks Tagged
    • Ref Manager
    • RIS
    • Zotero
    Request Permissions
    Share
    KNOTTED1 Cofactors, BLH12 and BLH14, Regulate Internode Patterning and Vein Anastomosis in Maize
    Katsutoshi Tsuda, Maria-Jazmin Abraham-Juarez, Akiteru Maeno, Zhaobin Dong, Dale Aromdee, Robert Meeley, Toshihiko Shiroishi, Ken-ichi Nonomura, Sarah Hake
    The Plant Cell May 2017, 29 (5) 1105-1118; DOI: 10.1105/tpc.16.00967
    del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
    • Tweet Widget
    • Facebook Like
    • Google Plus One

    Jump to section

    • Article
      • Abstract
      • INTRODUCTION
      • RESULTS
      • DISCUSSION
      • METHODS
      • Acknowledgments
      • AUTHOR CONTRIBUTIONS
      • Footnotes
      • References
    • Figures & Data
    • Info & Metrics
    • PDF

    In this issue

    The Plant Cell: 29 (5)
    The Plant Cell
    Vol. 29, Issue 5
    May 2017
    • Table of Contents
    • Table of Contents (PDF)
    • Cover (PDF)
    • About the Cover
    • Index by author
    View this article with LENS

    More in this TOC Section

    • Abscisic Acid-Triggered Persulfidation of the Cys Protease ATG4 Mediates Regulation of Autophagy by Sulfide
    • Temporal Regulation of the Metabolome and Proteome in Photosynthetic and Photorespiratory Pathways Contributes to Maize Heterosis
    • Chloroplast Chaperonin-Mediated Targeting of a Thylakoid Membrane Protein
    Show more RESEARCH ARTICLES

    Similar Articles

    Our Content

    • Home
    • Current Issue
    • Plant Cell Preview
    • Archive
    • Teaching Tools in Plant Biology
    • Plant Physiology
    • Plant Direct
    • Plantae
    • ASPB

    For Authors

    • Instructions
    • Submit a Manuscript
    • Editorial Board and Staff
    • Policies
    • Recognizing our Authors

    For Reviewers

    • Instructions
    • Peer Review Reports
    • Journal Miles
    • Transfer of reviews to Plant Direct
    • Policies

    Other Services

    • Permissions
    • Librarian resources
    • Advertise in our journals
    • Alerts
    • RSS Feeds
    • Contact Us

    Copyright © 2021 by The American Society of Plant Biologists

    Powered by HighWire