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PWWP-DOMAIN INTERACTOR OF POLYCOMBS1 Interacts with Polycomb-Group Proteins and Histones and Regulates Arabidopsis Flowering and Development

Mareike L. Hohenstatt, Pawel Mikulski, Olga Komarynets, Constanze Klose, Ina Kycia, Albert Jeltsch, Sara Farrona, Daniel Schubert
Mareike L. Hohenstatt
Institute for Genetics, Heinrich-Heine-University, 40225 Duesseldorf, Germany
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Pawel Mikulski
Institute for Genetics, Heinrich-Heine-University, 40225 Duesseldorf, GermanyInstitute of Biology, Freie Universität, 14195 Berlin, Germany
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Olga Komarynets
Institute for Genetics, Heinrich-Heine-University, 40225 Duesseldorf, GermanyUniversité de Genève, 1205 Geneva, Switzerland
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Constanze Klose
Institute for Genetics, Heinrich-Heine-University, 40225 Duesseldorf, Germany
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Ina Kycia
Institute for Biochemistry, University of Stuttgart, 70174 Stuttgart, Germany
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Albert Jeltsch
Institute for Biochemistry, University of Stuttgart, 70174 Stuttgart, Germany
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  • ORCID record for Albert Jeltsch
Sara Farrona
Institute for Genetics, Heinrich-Heine-University, 40225 Duesseldorf, GermanyPlant and AgriBiosciences Centre, National University Ireland, Galway H91 TK33, Ireland
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  • For correspondence: sara.farrona@nuigalway.iedan.schubert@fu-berlin.de
Daniel Schubert
Institute for Genetics, Heinrich-Heine-University, 40225 Duesseldorf, GermanyInstitute of Biology, Freie Universität, 14195 Berlin, Germany
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  • ORCID record for Daniel Schubert
  • For correspondence: sara.farrona@nuigalway.iedan.schubert@fu-berlin.de

Published January 2018. DOI: https://doi.org/10.1105/tpc.17.00117

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  • © 2018 American Society of Plant Biologists. All rights reserved.

Abstract

Polycomb-group (PcG) proteins mediate epigenetic gene regulation by setting H3K27me3 via Polycomb Repressive Complex 2 (PRC2). In plants, it is largely unclear how PcG proteins are recruited to their target genes. Here, we identified the PWWP-DOMAIN INTERACTOR OF POLYCOMBS1 (PWO1) protein, which interacts with all three Arabidopsis thaliana PRC2 histone methyltransferases and is required for maintaining full H3 occupancy at several Arabidopsis genes. PWO1 localizes and recruits CURLY LEAF to nuclear speckles in Nicotiana benthamiana nuclei, suggesting a role in spatial organization of PcG regulation. PWO1 belongs to a gene family with three members having overlapping activities: pwo1 pwo2 pwo3 triple mutants are seedling lethal and show shoot and root meristem arrest, while pwo1 single mutants are early flowering. Interestingly, the PWWP domain of PWO1 confers binding to histones, which is reduced by a point mutation in a highly conserved residue of this domain and blocked by phosphorylation of H3S28. PWO1 carrying this mutation is not able to fully complement the pwo1 pwo2 pwo3 triple mutant, indicating the requirement of this domain for PWO1 in vivo activity. Thus, the PWO family may present a novel class of histone readers that are involved in recruiting PcG proteins to subnuclear domains and in promoting Arabidopsis development.

  • Received February 10, 2017.
  • Revised November 28, 2017.
  • Accepted January 9, 2018.
  • Published January 12, 2018.
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PWWP-DOMAIN INTERACTOR OF POLYCOMBS1 Interacts with Polycomb-Group Proteins and Histones and Regulates Arabidopsis Flowering and Development
Mareike L. Hohenstatt, Pawel Mikulski, Olga Komarynets, Constanze Klose, Ina Kycia, Albert Jeltsch, Sara Farrona, Daniel Schubert
The Plant Cell Jan 2018, 30 (1) 117-133; DOI: 10.1105/tpc.17.00117

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PWWP-DOMAIN INTERACTOR OF POLYCOMBS1 Interacts with Polycomb-Group Proteins and Histones and Regulates Arabidopsis Flowering and Development
Mareike L. Hohenstatt, Pawel Mikulski, Olga Komarynets, Constanze Klose, Ina Kycia, Albert Jeltsch, Sara Farrona, Daniel Schubert
The Plant Cell Jan 2018, 30 (1) 117-133; DOI: 10.1105/tpc.17.00117
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The Plant Cell: 30 (1)
The Plant Cell
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