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S-Locus F-Box Proteins Are Solely Responsible for S-RNase-Based Self-Incompatibility of Petunia Pollen

Linhan Sun, Justin S. Williams, Shu Li, Lihua Wu, Wasi A. Khatri, Patrick G. Stone, Matthew D. Keebaugh, Teh-hui Kao
Linhan Sun
Intercollege Graduate Degree Program in Plant Biology, Pennsylvania State University, University Park, Pennsylvania 16802
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Justin S. Williams
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802
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Shu Li
Intercollege Graduate Degree Program in Plant Biology, Pennsylvania State University, University Park, Pennsylvania 16802
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Lihua Wu
Intercollege Graduate Degree Program in Plant Biology, Pennsylvania State University, University Park, Pennsylvania 16802
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Wasi A. Khatri
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802
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Patrick G. Stone
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802
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Matthew D. Keebaugh
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802
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Teh-hui Kao
Intercollege Graduate Degree Program in Plant Biology, Pennsylvania State University, University Park, Pennsylvania 16802Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802
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  • For correspondence: txk3@psu.edu

Published December 2018. DOI: https://doi.org/10.1105/tpc.18.00615

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  • © 2018 American Society of Plant Biologists. All rights reserved.

Abstract

Self-incompatibility (SI) in Petunia is regulated by a polymorphic S-locus. For each S-haplotype, the S-locus contains a pistil-specific S-RNase gene and multiple pollen-specific S-locus F-box (SLF) genes. Both gain-of-function and loss-of-function experiments have shown that S-RNase alone regulates pistil specificity in SI. Gain-of-function experiments on SLF genes suggest that the entire suite of encoded proteins constitute the pollen specificity determinant. However, clear-cut loss-of-function experiments must be performed to determine if SLF proteins are essential for SI of pollen. Here, we used CRISPR/Cas9 to generate two frame-shift indel alleles of S2-SLF1 (SLF1 of S2-haplotype) in S2S3 plants of P. inflata and examined the effect on the SI behavior of S2 pollen. In the absence of a functional S2-SLF1, S2 pollen was either rejected by or remained compatible with pistils carrying one of eight normally compatible S-haplotypes. All results are consistent with interaction relationships between the 17 SLF proteins of S2-haplotype and these eight S-RNases that had been determined by gain-of-function experiments performed previously or in this work. Our loss-of-function results provide definitive evidence that SLF proteins are solely responsible for SI of pollen, and they reveal their diverse and complex interaction relationships with S-RNases to maintain SI while ensuring cross-compatibility.

  • Received August 15, 2018.
  • Revised October 9, 2018.
  • Accepted October 25, 2018.
  • Published October 29, 2018.
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S-Locus F-Box Proteins Are Solely Responsible for S-RNase-Based Self-Incompatibility of Petunia Pollen
Linhan Sun, Justin S. Williams, Shu Li, Lihua Wu, Wasi A. Khatri, Patrick G. Stone, Matthew D. Keebaugh, Teh-hui Kao
The Plant Cell Dec 2018, 30 (12) 2959-2972; DOI: 10.1105/tpc.18.00615

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S-Locus F-Box Proteins Are Solely Responsible for S-RNase-Based Self-Incompatibility of Petunia Pollen
Linhan Sun, Justin S. Williams, Shu Li, Lihua Wu, Wasi A. Khatri, Patrick G. Stone, Matthew D. Keebaugh, Teh-hui Kao
The Plant Cell Dec 2018, 30 (12) 2959-2972; DOI: 10.1105/tpc.18.00615
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Extras

  • First author profile: Linhan Sun
  • In Brief: Self Control: SLF Proteins are Essential for Preventing Self-Fertilization in Petunia

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The Plant Cell: 30 (12)
The Plant Cell
Vol. 30, Issue 12
Dec 2018
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