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Shivaprasad, P.V., Chen, H.-M., Patel, K., Bond, D.M., Santos, B.A.C.M., and Baulcombe, D.C. (2012). A MicroRNA Superfamily Regulates Nucleotide Binding Site–Leucine-Rich Repeats and Other mRNAs. Plant Cell 24: 859–874 10.1105/tpc.111.095380.
The authors of the above article requested publication of a corrected Figure 2B. as shown below.
Source data 1. RNA gel blot-analysis of tomato miR482 isoforms and cross-hybridizing homologs in different species. All hybridizations the were performed using one membrane that was serially hybridized with different probes. The order of hybridizations, with date of scanning, is shown. AtmiR472 probe was used twice for hybridization of the same blot, as the signals in tomato lanes in the first blot obtained on 19th July 2011 were not clear and required reconfirmation. An exposure time for each blot is given in red.
In Figure 2, the authors assayed miR482 levels in Solanum genera members and other species. In order to verify next generation sequencing data (presented in Figure 2A) the authors used northern hybridization (Figure 2B) and, in one blot, the authors probed for Arabidopsis miR472 that has sequence similarity to tomato miR482. The aim was to find out whether this probe picked up a signal in the various plant species that are named in the figure.
Abundance of miR2118/482 Superfamily Members in Different Plants.
(B) RNA gel blot analysis of tomato miR482 isoforms and cross-hybridizing homologs in different species. Fifteen micrograms of total RNA from young seedlings was used for analysis of each sample. Total RNA was electrophoresed in a 15% polyacrylamide gel, transferred to membrane, and probed with corresponding DNA oligonucleotides (see Supplemental Table 1 online) labeled with [γ-32P]ATP. U6 serves as loading control. M, decade (Ambion) size marker.
In Figure 2B, for the top row corresponding to the probe for Arabidopsis miR472, multiple image layers were mistakenly included in the published figure, which led to the obscuration of faint bands in lanes corresponding to M82 and S. pennelli. The correct image is shown here with faint signals for M82 and S. pennelli. With this correction the statement in the text that “A probe for Arabidopsis miR472 hybridized with an RNA in potato but only weakly with tomato miRNAs” now matches the presented image.
Source data 2. RNA gel blot-analysis of tomato miR482 isoforms and cross-hybridizing homologs in S. tuberosum and Poplar. All hybridizations were performed using one membrane that was serially hybridized with different probes after stripping.
This figure is the only reference to Arabidopsis miR472 in the manuscript and all other results and conclusions of the manuscript remain unchanged. All authors have agreed to this correction.
Editor's note: The corrected figure and accompanying text were reviewed by members of The Plant Cell editorial board.
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