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A DNA Methylation Reader–Chaperone Regulator–Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress

Jie Wang, Nan Nan, Ning Li, Yutong Liu, Tian-Jing Wang, Inhwan Hwang, Bao Liu, Zheng-Yi Xu
Jie Wang
aKey Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, People’s Republic of China
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Nan Nan
aKey Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, People’s Republic of China
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Ning Li
aKey Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, People’s Republic of China
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Yutong Liu
aKey Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, People’s Republic of China
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Tian-Jing Wang
aKey Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, People’s Republic of China
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Inhwan Hwang
bDepartment of Life Sciences, Pohang University of Science and Technology, Pohang 37673, Korea
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Bao Liu
aKey Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, People’s Republic of China
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Zheng-Yi Xu
aKey Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, People’s Republic of China
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  • For correspondence: xuzy100@nenu.edu.cn

Published November 2020. DOI: https://doi.org/10.1105/tpc.20.00301

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  • © 2020 American Society of Plant Biologists. All rights reserved.

Abstract

Irrigated lands are increasingly salinized, which adversely affects agricultural productivity. To respond to high sodium (Na+) concentrations, plants harbor multiple Na+ transport systems. Rice (Oryza sativa) HIGH-AFFINITY POTASSIUM (K+) TRANSPORTER1;5 (OsHKT1;5), a Na+-selective transporter, maintains K+/Na+ homeostasis under salt stress. However, the mechanism regulating OsHKT1;5 expression remains unknown. Here, we present evidence that a protein complex consisting of rice BCL-2-ASSOCIATED ATHANOGENE4 (OsBAG4), OsMYB106, and OsSUVH7 regulates OsHKT1;5 expression in response to salt stress. We isolated a salt stress–sensitive mutant, osbag4-1, that showed significantly reduced OsHKT1;5 expression and reduced K+ and elevated Na+ levels in shoots. Using comparative interactomics, we isolated two OsBAG4-interacting proteins, OsMYB106 (a MYB transcription factor) and OsSUVH7 (a DNA methylation reader), that were crucial for OsHKT1;5 expression. OsMYB106 and OsSUVH7 bound to the MYB binding cis-element (MYBE) and the miniature inverted-repeat transposable element (MITE) upstream of the MYBE, respectively, in the OsHKT1;5 promoter. OsBAG4 functioned as a bridge between OsSUVH7 and OsMYB106 to facilitate OsMYB106 binding to the consensus MYBE in the OsHKT1;5 promoter, thereby activating the OsHKT1;5 expression. Elimination of the MITE or knockout of OsMYB106 or OsSUVH7 decreased OsHKT1;5 expression and increased salt sensitivity. Our findings reveal a transcriptional complex, consisting of a DNA methylation reader, a chaperone regulator, and a transcription factor, that collaboratively regulate OsHKT1;5 expression during salinity stress.

  • Received April 16, 2020.
  • Revised August 31, 2020.
  • Accepted September 13, 2020.
  • Published September 15, 2020.
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A DNA Methylation Reader–Chaperone Regulator–Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress
Jie Wang, Nan Nan, Ning Li, Yutong Liu, Tian-Jing Wang, Inhwan Hwang, Bao Liu, Zheng-Yi Xu
The Plant Cell Nov 2020, 32 (11) 3535-3558; DOI: 10.1105/tpc.20.00301

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A DNA Methylation Reader–Chaperone Regulator–Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress
Jie Wang, Nan Nan, Ning Li, Yutong Liu, Tian-Jing Wang, Inhwan Hwang, Bao Liu, Zheng-Yi Xu
The Plant Cell Nov 2020, 32 (11) 3535-3558; DOI: 10.1105/tpc.20.00301
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  • First author profile: Jie Wang
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The Plant Cell: 32 (11)
The Plant Cell
Vol. 32, Issue 11
Nov 2020
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