Skip to main content

Main menu

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Cell
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Cell

Advanced Search

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Follow PlantCell on Twitter
  • Visit PlantCell on Facebook
  • Visit Plantae
In BriefIN BRIEF
Open Access

Remodeling Flowering: CHROMATIN REMODELING4 Promotes the Floral Transition

Hanna Hõrak
Hanna Hõrak
Institute of TechnologyUniversity of Tartu, Estonia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Hanna Hõrak

Published May 2020. DOI: https://doi.org/10.1105/tpc.20.00196

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading
  • © 2020 American Society of Plant Biologists. All rights reserved.

Plants that fail to flower at the right time will lose out on seed production. Hence, the transition from vegetative growth to the reproductive phase depends strongly on environmental signals. Cold winter temperatures and long days are seasonal cues that stimulate flowering through vernalization and photoperiodic pathways, whereas high ambient temperatures also promote floral transition (Srikanth and Schmid, 2011). As endogenous signals, high levels of gibberellic acids (GAs; Mutasa-Göttgens and Hedden, 2009) and increasing plant age (Wang, 2014) lead to flowering in the absence of environmental triggers. In this issue, Sang et al. (2020) took an elegant approach to identify genes that act in the endogenous pathways for floral transition. They constructed a quintuple mutant deficient in induction of flowering by environmental signals and mutagenized it further to screen for endogenous regulators of flowering, identifying CHROMATIN REMODELING4 (CHR4) as a positive regulator of floral transition.

The quintuple mutant svp-41 flc-3 ft-10 tsf-1 soc1-2 had impaired flowering responses to long-day conditions, high temperature, and GA treatment, indicating that floral transition occurs via endogenous signals in these plants. RNA sequencing from samples of shoot apices at different time points after sowing showed higher mRNA levels of known endogenous flowering regulators in the quintuple mutant, such as SQUAMOSA PROMOTER BINDING PROTEIN-LIKE genes (SPLs) that promote flowering through the age- and GA-dependent pathways. Mutagenesis of the quintuple mutant and subsequent screening for late flowering led to the identification of two mutant lines named qem1 and qem2.

Fast-isogenic mapping and complementation analysis showed that the late-flowering phenotype in qem1 was caused by a mutation in the GA20ox2 gene that is involved in GA biosynthesis. Together with the higher GA20ox2 and SPL mRNA levels in the quintuple mutant compared with control plants, these data suggest that the GA pathway is involved in the early flowering of the quintuple mutant.

The mutation causing late flowering of qem2 was localized to CHR4, so the authors analyzed the phenotypes of qem2 side by side with the quintuple mutant and compared the chr4-2 mutant with respective wild-type Col-0. Plants deficient in CHR4 function had faster leaf production in later rosette growth stages, more cauline leaves, and larger shoot apical meristems, whereas all these phenotypes were stronger in the quintuple mutant background. Plants with the chr4 mutant alleles bolted earlier but took longer to flower after bolting. The authors suggest that the accelerated transition from vegetative phase to bolting in chr4 mutants could be caused by higher SPL15 expression levels. Both in qem2 and chr4-2, formation of the floral primordia was delayed, supporting an important role for CHR4 in establishing floral meristem identity.

CHR4-interacting proteins were identified by immunoprecipitation and included several transcription factors of the MADS, SPL, and AP2 families that regulate the floral transition and floral meristem identity, linking CHR4 to known regulators of flowering. Plants with chr4 alleles had alterations in mRNA levels of several genes involved in floral transition but also had different patterns of histone methylation. Genomic regions with increased or reduced levels of histone methylation were found in chr4 and were associated with known floral regulators such as miR156D and SPL15 that affect flowering in the age pathway and with genes involved in hormonal regulation through GAs, auxins, and cytokinins. The changed histone modification patterns and expression levels of components of the endogenous GA- and age-dependent pathways of floral induction, such as different SPLs; the various interaction partners of CHR4 suggest that CHR4 promotes flowering through several complexes and pathways (see figure). A further challenge lies in understanding how CHR4 achieves its manifold effects on gene expression.

Figure1
  • Download figure
  • Open in new tab
  • Download powerpoint

CHR4 Is a Positive Regulator of the Floral Transition.

Flowering is induced by environmental triggers and by age and GA endogenously. CHR4 affects histone methylation patterns and mRNA levels of genes involved in regulation of the floral transition by endogenous pathways.

Footnotes

  • www.plantcell.org/cgi/doi/10.1105/tpc.20.00196

  • ↵[OPEN] Articles can be viewed without a subscription.

References

  1. ↵
    1. Mutasa-Göttgens, E.,
    2. Hedden, P.
    (2009). Gibberellin as a factor in floral regulatory networks. J. Exp. Bot. 60: 1979–1989.
    OpenUrlCrossRefPubMed
  2. ↵
    1. Sang, Q.,
    2. Pajoro, A.,
    3. Sun, H.,
    4. Song, B.,
    5. Yang, X.,
    6. Stolze, S.-C.,
    7. Andrés, F.,
    8. Schneeberger, K.,
    9. Nakagami, H.,
    10. Coupland, G.
    (2020). Mutagenesis of a quintuple mutant impaired in environmental responses reveals roles for CHROMATIN REMODELING4 in Arabidopsis floral transition. Plant Cell 32: 1479–1500.
    OpenUrlAbstract/FREE Full Text
  3. ↵
    1. Srikanth, A.,
    2. Schmid, M.
    (2011). Regulation of flowering time: All roads lead to Rome. Cell. Mol. Life Sci. 68: 2013–2037.
    OpenUrlCrossRefPubMed
  4. ↵
    1. Wang, J.-W.
    (2014). Regulation of flowering time by the miR156-mediated age pathway. J. Exp. Bot. 65: 4723–4730.
    OpenUrlCrossRefPubMed
PreviousNext
Back to top

Table of Contents

Print
Download PDF
Email Article

Thank you for your interest in spreading the word on Plant Cell.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Remodeling Flowering: CHROMATIN REMODELING4 Promotes the Floral Transition
(Your Name) has sent you a message from Plant Cell
(Your Name) thought you would like to see the Plant Cell web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Remodeling Flowering: CHROMATIN REMODELING4 Promotes the Floral Transition
Hanna Hõrak
The Plant Cell May 2020, 32 (5) 1346-1347; DOI: 10.1105/tpc.20.00196

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
Remodeling Flowering: CHROMATIN REMODELING4 Promotes the Floral Transition
Hanna Hõrak
The Plant Cell May 2020, 32 (5) 1346-1347; DOI: 10.1105/tpc.20.00196
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

In this issue

The Plant Cell: 32 (5)
The Plant Cell
Vol. 32, Issue 5
May 2020
  • Table of Contents
  • Table of Contents (PDF)
  • Cover (PDF)
  • About the Cover
  • Index by author
View this article with LENS

More in this TOC Section

  • Hold Me, Fold Me...or Not!
  • Slice and Dice: DCL2 Mediates the Production of 22-Nucleotide siRNAs that Influence Trait Variation in Soybean
  • How to Eat One’s Feelings: Autophagy and Phosphatidylinositol 3-Phosphate
Show more IN BRIEF

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Cell Preview
  • Archive
  • Teaching Tools in Plant Biology
  • Plant Physiology
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Peer Review Reports
  • Journal Miles
  • Transfer of reviews to Plant Direct
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds
  • Contact Us

Copyright © 2021 by The American Society of Plant Biologists

Powered by HighWire