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Spatiotemporal Restriction of FUSCA3 Expression by Class I BPCs Promotes Ovule Development and Coordinates Embryo and Endosperm Growth

Jian Wu, Deka Mohamed, Sebastian Dowhanik, Rosanna Petrella, Veronica Gregis, Jingru Li, Lin Wu, Sonia Gazzarrini
Jian Wu
aBeijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193, China
bDepartment of Biological Sciences, University of Toronto Scarborough, Ontario M1C 1A4, Canada
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Deka Mohamed
bDepartment of Biological Sciences, University of Toronto Scarborough, Ontario M1C 1A4, Canada
cDepartment of Cell and Systems Biology, University of Toronto, Ontario M5S 3G5, Canada
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Sebastian Dowhanik
bDepartment of Biological Sciences, University of Toronto Scarborough, Ontario M1C 1A4, Canada
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Rosanna Petrella
dDipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133, Milano, Italy
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Veronica Gregis
dDipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133, Milano, Italy
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Jingru Li
aBeijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193, China
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Lin Wu
bDepartment of Biological Sciences, University of Toronto Scarborough, Ontario M1C 1A4, Canada
eChongqing Key Laboratory of Economic Plant Biotechnology, Institute of Special Plants, Chongqing University of Arts and Sciences, Yongchuan, Chongqing 402160, China
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Sonia Gazzarrini
bDepartment of Biological Sciences, University of Toronto Scarborough, Ontario M1C 1A4, Canada
cDepartment of Cell and Systems Biology, University of Toronto, Ontario M5S 3G5, Canada
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  • For correspondence: gazzarrini@utsc.utoronto.ca

Published June 2020. DOI: https://doi.org/10.1105/tpc.19.00764

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  • © 2020 American Society of Plant Biologists. All rights reserved.

Abstract

Spatiotemporal regulation of gene expression is critical for proper developmental timing in plants and animals. The transcription factor FUSCA3 (FUS3) regulates developmental phase transitions by acting as a link between hormonal pathways in Arabidopsis (Arabidopsis thaliana). However, the mechanisms governing its spatiotemporal expression pattern are poorly understood. Here, we show that FUS3 is repressed in the ovule integuments and seed endosperm. FUS3 repression requires class I BASIC PENTACYSTEINE (BPC) proteins, which directly bind GA/CT cis-elements in FUS3 and restrict its expression pattern. During vegetative and reproductive development, FUS3 derepression in bpc1-1 bpc2 (bpc1/2) double mutant or misexpression in ProML1:FUS3 lines causes dwarf plants carrying defective flowers and aborted ovules. After fertilization, ectopic FUS3 expression in bpc1/2 endosperm or ProML1:FUS3 endosperm and endothelium increases endosperm nuclei proliferation and seed size, causing delayed or arrested embryo development. These phenotypes are rescued in bpc1/2 fus3-3. Finally, class I BPCs interact with FIS-PRC2 (FERTILIZATION-INDEPENDENT SEED-Polycomb Repressive Complex2), which represses FUS3 in the endosperm during early seed development. We propose that BPC1 and 2 promote the transition from reproductive to seed development by repressing FUS3 in ovule integuments. After fertilization, BPC1 and 2 and FIS-PRC2 repress FUS3 in the endosperm to coordinate early endosperm and embryo growth.

  • Received December 2, 2019.
  • Revised March 10, 2020.
  • Accepted April 3, 2020.
  • Published April 7, 2020.
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Spatiotemporal Restriction of FUSCA3 Expression by Class I BPCs Promotes Ovule Development and Coordinates Embryo and Endosperm Growth
Jian Wu, Deka Mohamed, Sebastian Dowhanik, Rosanna Petrella, Veronica Gregis, Jingru Li, Lin Wu, Sonia Gazzarrini
The Plant Cell Jun 2020, 32 (6) 1886-1904; DOI: 10.1105/tpc.19.00764

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Spatiotemporal Restriction of FUSCA3 Expression by Class I BPCs Promotes Ovule Development and Coordinates Embryo and Endosperm Growth
Jian Wu, Deka Mohamed, Sebastian Dowhanik, Rosanna Petrella, Veronica Gregis, Jingru Li, Lin Wu, Sonia Gazzarrini
The Plant Cell Jun 2020, 32 (6) 1886-1904; DOI: 10.1105/tpc.19.00764
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The Plant Cell: 32 (6)
The Plant Cell
Vol. 32, Issue 6
Jun 2020
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