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Arabidopsis ROOT PHOTOTROPISM2 Is a Light-Dependent Dynamic Modulator of Phototropin1

Taro Kimura, Tomoko Tsuchida-Mayama, Hirotatsu Imai, Koji Okajima, Kosuke Ito, Tatsuya Sakai
Taro Kimura
aGraduate School of Science and Technology, Niigata University, Niigata-shi, Niigata, 950-2181, Japan
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  • ORCID record for Taro Kimura
Tomoko Tsuchida-Mayama
bRIKEN Plant Science Center, Yokohama, Kanagawa 230-0045, Japan
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  • ORCID record for Tomoko Tsuchida-Mayama
Hirotatsu Imai
aGraduate School of Science and Technology, Niigata University, Niigata-shi, Niigata, 950-2181, Japan
cResearch Fellow of Japan Society for the Promotion of Science, Kojimachi Business Center Building, Chiyoda-ku, Tokyo 102-0083, Japan
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Koji Okajima
dDepartment of Physics, Keio University, 3-14-1, Hiyoshi, Kouhoku-ku, Yokohama, Kanagawa 223-8522, Japan
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Kosuke Ito
aGraduate School of Science and Technology, Niigata University, Niigata-shi, Niigata, 950-2181, Japan
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Tatsuya Sakai
aGraduate School of Science and Technology, Niigata University, Niigata-shi, Niigata, 950-2181, Japan
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  • ORCID record for Tatsuya Sakai
  • For correspondence: tsakai@gs.niigata-u.ac.jp

Published June 2020. DOI: https://doi.org/10.1105/tpc.19.00926

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  • © 2020 American Society of Plant Biologists. All rights reserved.

Abstract

The Arabidopsis (Arabidopsis thaliana) blue light photoreceptor phototropin1 (phot1) is a blue light–activated Ser/Thr protein kinase that mediates various light responses, including phototropism. The function of phot1 in hypocotyl phototropism is dependent on the light induction of ROOT PHOTOTROPISM2 (RPT2) proteins within a broad range of blue light intensities. It is not yet known however how RPT2 contributes to the photosensory adaptation of phot1 to high intensity blue light and the phototropic responses under bright light conditions. We show that RPT2 suppresses the activity of phot1 and demonstrate that RPT2 binds to the PHOT1 light, oxygen or voltage sensing1 (LOV1) domain that is required for its high photosensitivity. Our biochemical analyses revealed that RPT2 inhibits autophosphorylation of phot1, suggesting that it suppresses the photosensitivity and/or kinase activity of phot1 through the inhibition of LOV1 function. We found that RPT2 proteins are degraded via a ubiquitin-proteasome pathway when phot1 is inactive and are stabilized under blue light in a phot1-dependent manner. We propose that RPT2 is a molecular rheostat that maintains a moderate activation level of phot1 under any light intensity conditions.

  • Received December 2, 2019.
  • Revised January 22, 2020.
  • Accepted March 17, 2020.
  • Published March 25, 2020.
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Arabidopsis ROOT PHOTOTROPISM2 Is a Light-Dependent Dynamic Modulator of Phototropin1
Taro Kimura, Tomoko Tsuchida-Mayama, Hirotatsu Imai, Koji Okajima, Kosuke Ito, Tatsuya Sakai
The Plant Cell Jun 2020, 32 (6) 2004-2019; DOI: 10.1105/tpc.19.00926

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Arabidopsis ROOT PHOTOTROPISM2 Is a Light-Dependent Dynamic Modulator of Phototropin1
Taro Kimura, Tomoko Tsuchida-Mayama, Hirotatsu Imai, Koji Okajima, Kosuke Ito, Tatsuya Sakai
The Plant Cell Jun 2020, 32 (6) 2004-2019; DOI: 10.1105/tpc.19.00926
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The Plant Cell: 32 (6)
The Plant Cell
Vol. 32, Issue 6
Jun 2020
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