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Ho, W.W.H., Weigel, D. (2014). Structural Features Determining Flower-Promoting Activity of Arabidopsis FLOWERING LOCUS T. Plant Cell 26: 552–564.
During the assembly of Supplemental Figure 7 and Figure 8, six luciferase images of individual leaves were inadvertently duplicated. These images served to illustrate the strength of interactions between FT and TFL1 proteins and their mutant variants with GRF and TCP proteins, as measured qualitatively in split luciferase assays and in support of independent, quantitative yeast-two-hybrid experiments. The authors went back to the original photographs, and prepared a corrected version that contains the correct images for the six duplicated leaves. Figure 8 shows a subset of the leaves shown in Supplemental Figure 7; corrections for both figures are shown below.
The figures are referred to twice in the text:
“As expected, FT, TFL1, the TFL-1–like FT mutant Q140K as well as the FT mutant Q140D, which is predicted to have the same surface charge as native FT, all interacted similarly well with several 14-3-3/GRF proteins and with FD (Figures 7 and 8; Supplemental Figure 7).”
(original). Bimolecular luciferase complementation assays.
Young but fully expanded 4-week-old N. benthamiana leaves were strictly chosen for infiltration. Experiments were repeated twice. Complete set of assay results is shown in Supplemental Figure 7.
Note added in correction: Leaves surrounded by heavy lines and numbered in gray indicate images that were correctly assigned. Leaves surrounded by light lines and numbered in red indicate images that were duplicated and which are replaced in the corrected version.
(original). Bimolecular luciferase complementation assays.
Young but fully expanded 4-week-old N. benthamiana leaves were strictly chosen for infiltration. Experiments were repeated twice. A selection of TCP interactions is shown in Figure 8.
Note added in correction: Leaves surrounded by heavy lines and numbered in gray indicate images that were correctly assigned. Leaves surrounded by light lines and numbered in red indicate images that were duplicated and which are replaced in the corrected version.
“The same trends were observed in qualitative assays using bimolecular luminescence complementation assays in N. benthamiana (Figure 8; Supplemental Figure 7).”
These descriptions of the experiments remain correct.
Versions of the original Supplemental Figure 7 and of the original Figure 8 in which the duplications are indicated are shown below. Leaves surrounded by heavy lines and numbered in gray indicate images that were correctly assigned. Leaves surrounded by light lines and numbered in red indicate images that were duplicated and which are replaced in the corrected versions of Supplemental Figure 7 and Figure 8.
(corrected). Bimolecular luciferase complementation assays.
Young but fully expanded 4-week-old N. benthamiana leaves were strictly chosen for infiltration. Experiments were repeated twice. Complete set of assay results is shown in Supplemental Figure 7.
Leaves surrounded by light lines and numbered in red indicate the images that were corrected compared to the original.
(corrected). Bimolecular luciferase complementation assays.
Young but fully expanded 4-week-old N. benthamiana leaves were strictly chosen for infiltration. Experiments were repeated twice. A selection of TCP interactions is shown in Figure 8.
Leaves surrounded by light lines and numbered in red indicate the images that were corrected compared to the original.
All the original photographs from which the leaves of all experiments were extracted, including a table with a key to the images, are available at https://datadryad.org/stash/dataset/doi:10.5061/dryad.h9w0vt4fh. The experiments affected by the duplication are highlighted in red in this table.
The authors apologize for these errors.
Note: This correction was reviewed by members of The Plant Cell editorial board. The authors are responsible for providing a complete listing and accurate explanations for all known errors or instances of inappropriate data handling or image manipulation associated with the original publication.
