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Zhao, J., Jiang, L., Che, G., Pan, Y., Li, Y., Hou, Y., Zhao, W., Zhong, Y., Ding, L., Yan, S., Sun, C., Liu, R., et al. (2019). A Functional Allele of CsFUL1 Regulates Fruit Length through Repressing CsSUP and Inhibiting Auxin Transport in Cucumber. Plant Cell 31: 1289–1307.
The authors of the above article request the correction of Figures 3A, 3D, 3F and 3G, Figure 4B, Figure 5B, Figure 6B, Figure 7A, Figure 8B, and Supplemental Figure 10B, as described below. The original results and conclusions are unaffected by these corrections. The authors apologize for any inconvenience caused to the readers. All authors have approved this correction.
In order to obtain fruits at the same developmental stage for length quantification, some of the fruits were imaged separately with a ruler and the images merged together during manuscript preparation. We corrected Figures 3A, 3D, 3F and 3G, Figure 4B, and Figure 7A by adding white lines between the separate images (corrected panels boxed in red for Figures 3, 4, and 5; corrected panel only shown for Figure 7). The figure legends and remaining figure panels are unchanged from the original version.
In the original Figure 5B, the same cucumber fruit was unintentionally imaged twice with different scales and mistakenly saved as different names (V/C and A-RNAi-6) during image processing. The corrected Figure 5B shows representative fruits of V/C and A-RNAi-6 transgenic lines; the replaced images are underlined in red. The figure legend and remaining figure panels are the same as the original version.
Source data for Figure 6 and Supplemental Figure 10.
Source data for Figure 6 and Supplemental Figure 10.
Heterologous Expression of CsFUL1 in Arabidopsis.
(A) to (E) Heterologous expression of CsFUL1A and CsFUL1C in ful-1 mutant Arabidopsis.(A) Representative silique images of 35S:CsFUL1A/ful-1 plants. (B) Scanning electron microscopic images of siliques in ful-1 and 35S:CsFUL1A/ful-1. Red lines indicate the replum zones. (C) RT-qPCR analysis of CsFUL1 in the 35S:CsFUL1A/ful-1 transgenic plants. (D) Representative images of fruits of 35S:CsFUL1C/ful-1 plants. (E) RT-qPCR analysis of CsFUL1 in 35S:CsFUL1C/ful-1 transgenic plants. Values are means ± se (n = 3).
(F) to (K) Overexpression of CsFUL1A and CsFUL1C in the wild-type Arabidopsis.
(F) and (G) Representative images of fruits of 35S:CsFUL1A plants (F) and 35S:CsFUL1C plants (G).
(H) and (I) RT-qPCR analysis of CsFUL1 in the 35S:CsFUL1A(H) and 35S:CsFUL1C(I) background.
(J) and (K) Statistical analyses of fruit length in the 35S:CsFUL1A(J) and 35S:CsFUL1C lines (K). Values are means ± se (n = 30 fruits). Ler, Landsberg erecta. Three independent samples from different plants were used for gene expression. Two-tailed Student's t tests were used to compare the transgenic lines and the corresponding controls (*P < 0.05; **P < 0.01). Bar = 1 mm in (A), (D), (F), and (G) and 100 µm in (B).
Phenotypic Characterization of 35S:CsFUL1 in Long-Fruited Inbred Line R1407 Harboring the A Allele.
(A) to (D) Representative fruits of three CsFUL1A and CsFUL1C overexpression lines at 10 DAA (A, C) and at maturity (>30 DAA) (B, D). Bars = 5 cm. V/C, empty vector/control plants.
(E) and (F) CsFUL1 expression in young fruits of CsFUL1A(E) and CsFUL1C overexpression lines (F). Three independent samples from different plants were used for expression analysis. Values are means ± se.
(G) and (H) Fruit length quantification of CsFUL1A(G) and CsFUL1C overexpression lines (H). Values are means ± se (n = 4 fruits from different plants).
(I) and (J) Longitudinal sections of the fruit pericarp at maturity in CsFUL1A overexpression lines. Bars = 200 µm.
(K) Cell area in the fruit pericarp in CsFUL1A overexpression lines. Values are means ± se (n = 3 pericarps from different fruits). Significance analysis was conducted with the two-tailed Student's t tests (*P < 0.05; **P < 0.01).
CsFUL1A-RNAi Resulted in Longer Fruits in Inbred Line R1407 with the A Allele.
(A) to (D) Representative fruits at 10 DAA ([A] and [C]) and at maturity ([B] and [D]) in CsFUL1A-RNAi and CsFUL1C-RNAi lines. Bars = 5 cm. V/C, empty vector/control plants.
(E) to (G) CsFUL1 and CsFUL2 expression in young fruits of CsFUL1A-RNAi plants ([E] and [F]) and CsFUL1C-RNAi plants (G) as detected by RT-qPCR. Three independent samples from different plants were used for expression analysis. Values are means ± se. V/C, empty vector/control plants.
(H) to (J) Fruit length at 10 DAA and at maturity. Values are means ± se (n = 4 fruits). V/C, empty vector/control plants.
(K) and (L) Longitudinal sections of fruits at maturity in control (K) and CsFUL1A-RNAi (L) lines. Bars = 200 µm. V/C, empty vector/control plants.
(M) Cell area in the fruit pericarp of CsFUL1A-RNAi plants. Values are means ± se (n = 3 pericarps). Significance analysis was conducted using two-tailed Student's t tests (*P < 0.05; **P < 0.01). V/C, empty vector/control plants.
CsFUL1A Inhibited Expression of CsSUP in Cucumber.
(B) CsFUL1A and CsFUL1C bound to CArG-box containing fragments of CsSUP in a Y1H assay, while the type II MADS-box protein CsCUM10 did not. Interaction of IND-AD with the PID-E box as a positive control and the empty AD and PID-E box as a negative control. AD, activation domain; E, exon; P, promoter.
CsFUL1A Downregulated CsPIN1 and CsPIN7 Transcription.
(A) Young fruits in CsFUL1A-OX and control lines at green bud (N1, N2-1), green-yellow bud (N2-2), yellow bud (N3), and flowering (N4) stages.
Protein Interactions Detected with Y2H and BiFC.
(B) Y2H assays showed CsFUL1A interacts with seven proteins including CsAGL20, while CsFUL1C does not interact with CsAGL20. No interactions were detected between CsFUL1A and CsFUL1C, CsFUL2, CsALC, or CsHEC3. No homodimer or heterodimer formed between CsFUL1A and CsFUL1C.
Y1H assay. (Supports Figure 7).
(B) The CsFUL1 A allele and CsFUL1 C allele bound to promoter fragments of CsPIN1 and CsPIN7 containing the CArG-box, but not to CsPID and CsARF12. CsCUM10 could not bind to these CArG-boxes. The numbers in the right corner indicate the basal concentration of AbA (Aureobasidin A), including 100 ng ·mL-1, 400 ng ·mL-1 and 500 ng ·mL-1.
Source data for Figure 6 and Supplemental Figure 10.
In the original Figure 6B and Supplemental Figure 10B, colony copy and paste errors occurred in the Y1H experiment, including that the two yeast colonies on the first row of Figure 6B were duplicates, and that control yeast images of CsFUL1A/P11 and CsFUL1A/P13 were repeatedly used three and two times, respectively, in Supplemental Figure 10B. We carefully checked the original yeast plate picture (shown with red circled colonies in images marked Source data) and corrected these two figures accordingly. The figure legend and remaining figure panels are unaltered from the original version. The replaced images in the corrected figures are underlined in red.
The same image processing mistake occurred in the top six rows of Figure 8B, including that yeast pictures of CsFUL1A-AD/BD were repeatedly used three times in the first two rows, and that colony images of CsFUL1A-BD/CsSEP2-AD and CsFUL1A-BD/CsSEP4-AD were repeated twice in rows 3-6. We traced back to the original yeast plate picture (shown with red circled colonies) and corrected Figure 8B accordingly. The figure legend and the rest of the figure panels are unaltered from the original version.
Editors' note: This correction was reviewed by members of The Plant Cell editorial board. The authors are responsible for providing a complete listing and accurate explanations for all known errors or instances of inappropriate data handling or image manipulation associated with the original publication.
