Skip to main content

Main menu

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Cell
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Cell

Advanced Search

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Follow PlantCell on Twitter
  • Visit PlantCell on Facebook
  • Visit Plantae
Research ArticleResearch Article
You have accessRestricted Access

Molecular Mechanism of Polysaccharide Acetylation by the Arabidopsis Xylan O-acetyltransferase XOAT1

Vladimir V. Lunin, Hsin-Tzu Wang, Vivek S. Bharadwaj, Markus Alahuhta, Maria J. Peña, Jeong-Yeh Yang, Stephanie A. Archer-Hartmann, Parastoo Azadi, Michael E. Himmel, Kelley W. Moremen, William S. York, Yannick J. Bomble, Breeanna R. Urbanowicz
Vladimir V. Lunin
aBioscience Center, National Renewable Energy Laboratory, Golden, Colorado 80401
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Vladimir V. Lunin
Hsin-Tzu Wang
bComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602
cDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Hsin-Tzu Wang
Vivek S. Bharadwaj
aBioscience Center, National Renewable Energy Laboratory, Golden, Colorado 80401
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Vivek S. Bharadwaj
Markus Alahuhta
aBioscience Center, National Renewable Energy Laboratory, Golden, Colorado 80401
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Markus Alahuhta
Maria J. Peña
bComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Maria J. Peña
Jeong-Yeh Yang
bComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jeong-Yeh Yang
Stephanie A. Archer-Hartmann
bComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Stephanie A. Archer-Hartmann
Parastoo Azadi
bComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Parastoo Azadi
Michael E. Himmel
aBioscience Center, National Renewable Energy Laboratory, Golden, Colorado 80401
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Michael E. Himmel
Kelley W. Moremen
bComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602
cDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kelley W. Moremen
William S. York
bComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602
cDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for William S. York
Yannick J. Bomble
aBioscience Center, National Renewable Energy Laboratory, Golden, Colorado 80401
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Yannick J. Bomble
  • For correspondence: yannick.bomble@nrel.gov breeanna@uga.edu
Breeanna R. Urbanowicz
bComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602
cDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Breeanna R. Urbanowicz
  • For correspondence: yannick.bomble@nrel.gov breeanna@uga.edu

Published July 2020. DOI: https://doi.org/10.1105/tpc.20.00028

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading
  • © 2020 American Society of Plant Biologists. All rights reserved.

Abstract

Xylans are a major component of plant cell walls. O-Acetyl moieties are the dominant backbone substituents of glucuronoxylan in dicots and play a major role in the polymer-polymer interactions that are crucial for wall architecture and normal plant development. Here, we describe the biochemical, structural, and mechanistic characterization of Arabidopsis (Arabidopsis thaliana) xylan O-acetyltransferase 1 (XOAT1), a member of the plant-specific Trichome Birefringence Like (TBL) family. Detailed characterization of XOAT1-catalyzed reactions by real-time NMR confirms that it exclusively catalyzes the 2-O-acetylation of xylan, followed by nonenzymatic acetyl migration to the O-3 position, resulting in products that are monoacetylated at both O-2 and O-3 positions. In addition, we report the crystal structure of the catalytic domain of XOAT1, which adopts a unique conformation that bears some similarities to the α/β/α topology of members of the GDSL-like lipase/acylhydrolase family. Finally, we use a combination of biochemical analyses, mutagenesis, and molecular simulations to show that XOAT1 catalyzes xylan acetylation through formation of an acyl-enzyme intermediate, Ac–Ser-216, by a double displacement bi-bi mechanism involving a Ser-His-Asp catalytic triad and unconventionally uses an Arg residue in the formation of an oxyanion hole.

  • Received January 16, 2020.
  • Revised April 14, 2020.
  • Accepted April 29, 2020.
  • Published April 30, 2020.
View Full Text

Log in using your username and password

Forgot your user name or password?

Pay Per Article - You may access this article (from the computer you are currently using) for 2 days for US$20.00

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

PreviousNext
Back to top

Table of Contents

Print
Download PDF
Email Article

Thank you for your interest in spreading the word on Plant Cell.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Molecular Mechanism of Polysaccharide Acetylation by the Arabidopsis Xylan O-acetyltransferase XOAT1
(Your Name) has sent you a message from Plant Cell
(Your Name) thought you would like to see the Plant Cell web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Molecular Mechanism of Polysaccharide Acetylation by the Arabidopsis Xylan O-acetyltransferase XOAT1
Vladimir V. Lunin, Hsin-Tzu Wang, Vivek S. Bharadwaj, Markus Alahuhta, Maria J. Peña, Jeong-Yeh Yang, Stephanie A. Archer-Hartmann, Parastoo Azadi, Michael E. Himmel, Kelley W. Moremen, William S. York, Yannick J. Bomble, Breeanna R. Urbanowicz
The Plant Cell Jul 2020, 32 (7) 2367-2382; DOI: 10.1105/tpc.20.00028

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
Molecular Mechanism of Polysaccharide Acetylation by the Arabidopsis Xylan O-acetyltransferase XOAT1
Vladimir V. Lunin, Hsin-Tzu Wang, Vivek S. Bharadwaj, Markus Alahuhta, Maria J. Peña, Jeong-Yeh Yang, Stephanie A. Archer-Hartmann, Parastoo Azadi, Michael E. Himmel, Kelley W. Moremen, William S. York, Yannick J. Bomble, Breeanna R. Urbanowicz
The Plant Cell Jul 2020, 32 (7) 2367-2382; DOI: 10.1105/tpc.20.00028
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Extras

  • First author profile: Vladimir Lunin
  • First author profile: Hsin-Tzu Wang
  • First author profile: Vivek Bharadwaj
  • First author profile: Markus Alahuhta

Jump to section

  • Article
    • Abstract
    • INTRODUCTION
    • RESULTS AND DISCUSSION
    • SUMMARY
    • METHODS
    • DIVE Curated Terms
    • Acknowledgments
    • AUTHOR CONTRIBUTIONS
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

In this issue

The Plant Cell: 32 (7)
The Plant Cell
Vol. 32, Issue 7
Jul 2020
  • Table of Contents
  • Table of Contents (PDF)
  • Cover (PDF)
  • About the Cover
  • Index by author
View this article with LENS

More in this TOC Section

  • Chloroplast Chaperonin-Mediated Targeting of a Thylakoid Membrane Protein
  • Ectopic Expression of the Transcriptional Regulator silky3 Causes Pleiotropic Meristem and Sex Determination Defects in Maize Inflorescences
  • SAUR17 and SAUR50 Differentially Regulate PP2C-D1 during Apical Hook Development and Cotyledon Opening in Arabidopsis
Show more RESEARCH ARTICLES

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Cell Preview
  • Archive
  • Teaching Tools in Plant Biology
  • Plant Physiology
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Peer Review Reports
  • Journal Miles
  • Transfer of reviews to Plant Direct
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds
  • Contact Us

Copyright © 2021 by The American Society of Plant Biologists

Powered by HighWire