Analysis of Leaf Sectors in the NCS6 Mitochondrial Mutant of Maize.

Abstract

The nonchromosomal stripe (NCS6) mutation of maize is a partial deletion of the mitochondrial cytochrome oxidase subunit 2 (Cox2) gene. The Cox2 deletion and a narrow yellow striping phenotype are inherited together in a maternal fashion. The striped plants are heteroplasmic for mutant and normal Cox2 genes. Only the mutant Cox2 gene is detected within the yellow stripes, whereas both normal and mutant forms of the gene are present in the green sectors of the NCS6 plants. In the green leaves of nonstriped relatives, only the normal Cox2 gene is found. Both the structure and functioning of the chloroplasts in the yellow leaf sectors of NCS6 plants are altered. The pleiotropic effects of the NCS6 mutation suggest that mitochondrial function is required for the development of photosynthetically competent chloroplasts.