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Abstract
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Isolated Plant Nuclei Nucleate Microtubule Assembly: The Nuclear Surface in Higher Plants Has Centrosome-like Activity.

V. Stoppin, M. Vantard, A. C. Schmit, A. M. Lambert
V. Stoppin
Institut de Biologie Moleculaire des Plantes, Centre National de la Recherche Scientifique, Universite Louis Pasteur, 12 rue du General Zimmer, F-67084 Strasbourg Cedex, France.
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M. Vantard
Institut de Biologie Moleculaire des Plantes, Centre National de la Recherche Scientifique, Universite Louis Pasteur, 12 rue du General Zimmer, F-67084 Strasbourg Cedex, France.
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A. C. Schmit
Institut de Biologie Moleculaire des Plantes, Centre National de la Recherche Scientifique, Universite Louis Pasteur, 12 rue du General Zimmer, F-67084 Strasbourg Cedex, France.
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A. M. Lambert
Institut de Biologie Moleculaire des Plantes, Centre National de la Recherche Scientifique, Universite Louis Pasteur, 12 rue du General Zimmer, F-67084 Strasbourg Cedex, France.
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Published August 1994. DOI: https://doi.org/10.1105/tpc.6.8.1099

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  • Copyright © 1994 by American Society of Plant Biologists

Abstract

In most eukaryotic cells, microtubules (MTs) are assembled at identified nucleating sites, such as centrosomes or spindle pole bodies. Higher plant cells do not possess such centrosome-like structures. Thus, the fundamental issues of where and how the intracellular plant MTs are nucleated remain highly debatable. A large body of evidence indicates that plant MTs emerge from the nuclear periphery. In this study, we developed an in vitro assay in which isolated maize nuclei nucleate MT assembly at a tubulin concentration (14 [mu]M of neurotubulin) that is not efficient for spontaneous MT assembly. No MT-stabilizing agents, such as taxol or dimethyl sulfoxide, were used. Our model provides evidence that the nuclear surface functions as a MT-nucleating site in higher plant cells. A monoclonal antibody raised against a pericentriolar antigen immunostained the surface of isolated nuclei, and a 100-kD polypeptide in 4 M urea-treated nuclear extracts was detected.

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Isolated Plant Nuclei Nucleate Microtubule Assembly: The Nuclear Surface in Higher Plants Has Centrosome-like Activity.
V. Stoppin, M. Vantard, A. C. Schmit, A. M. Lambert
The Plant Cell Aug 1994, 6 (8) 1099-1106; DOI: 10.1105/tpc.6.8.1099

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Isolated Plant Nuclei Nucleate Microtubule Assembly: The Nuclear Surface in Higher Plants Has Centrosome-like Activity.
V. Stoppin, M. Vantard, A. C. Schmit, A. M. Lambert
The Plant Cell Aug 1994, 6 (8) 1099-1106; DOI: 10.1105/tpc.6.8.1099
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Vol. 6, Issue 8
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