Skip to main content

Main menu

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Cell
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Cell

Advanced Search

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Follow PlantCell on Twitter
  • Visit PlantCell on Facebook
  • Visit Plantae
Abstract
You have accessRestricted Access

The tobacco homolog of mammalian calreticulin is present in protein complexes in vivo.

J Denecke, L E Carlsson, S Vidal, A S Höglund, B Ek, M J van Zeijl, K M Sinjorgo, E T Palva
J Denecke
Department of Molecular Genetics, Swedish University of Agricultural Sciences.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
L E Carlsson
Department of Molecular Genetics, Swedish University of Agricultural Sciences.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
S Vidal
Department of Molecular Genetics, Swedish University of Agricultural Sciences.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
A S Höglund
Department of Molecular Genetics, Swedish University of Agricultural Sciences.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
B Ek
Department of Molecular Genetics, Swedish University of Agricultural Sciences.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M J van Zeijl
Department of Molecular Genetics, Swedish University of Agricultural Sciences.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
K M Sinjorgo
Department of Molecular Genetics, Swedish University of Agricultural Sciences.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
E T Palva
Department of Molecular Genetics, Swedish University of Agricultural Sciences.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Published April 1995. DOI: https://doi.org/10.1105/tpc.7.4.391

  • Article
  • Info & Metrics
  • PDF
Loading
  • Copyright © 1995 by American Society of Plant Biologists

Abstract

The analysis of protein sorting signals responsible for the retention of reticuloplasmins (RPLs), a group of soluble proteins that reside in the lumen of the endoplasmic reticulum (ER), has revealed a structural similarity between mammalian and plant ER retention signals. We present evidence that the corresponding epitope is conserved in a vast family of soluble ER resident proteins. Microsequences of RPL60 and RPL90, two abundant members of this family, show high sequence similarity with mammalian calreticulin and endoplasmin. RPL60/calreticulin cofractionates and costains with the lumenal binding protein (BiP). Both proteins were detected in the nuclear envelope and the ER, and in mitotic cells in association with the spindle apparatus and the phragmoplast. Immunoprecipitation of proteins from in vivo-labeled cells demonstrated that RPL60/calreticulin is associated with other polypeptides in a stress- and ATP-dependent fashion. RPL60/calreticulin transcript levels increased rapidly in abundance during the proliferation of the secretory apparatus and the onset of hydrolase secretion in gibberellic acid-treated barley aleurone cells. This induction profile is identical to that of the well-characterized ER chaperones BiP and endoplasmin. However, expression patterns in response to different stress conditions as well as tissue-specific expression patterns indicate that these genes are differentially regulated and may not act in concert.

PreviousNext
Back to top

Table of Contents

Download PDF
Email Article

Thank you for your interest in spreading the word on Plant Cell.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
The tobacco homolog of mammalian calreticulin is present in protein complexes in vivo.
(Your Name) has sent you a message from Plant Cell
(Your Name) thought you would like to see the Plant Cell web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
The tobacco homolog of mammalian calreticulin is present in protein complexes in vivo.
J Denecke, L E Carlsson, S Vidal, A S Höglund, B Ek, M J van Zeijl, K M Sinjorgo, E T Palva
The Plant Cell Apr 1995, 7 (4) 391-406; DOI: 10.1105/tpc.7.4.391

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
The tobacco homolog of mammalian calreticulin is present in protein complexes in vivo.
J Denecke, L E Carlsson, S Vidal, A S Höglund, B Ek, M J van Zeijl, K M Sinjorgo, E T Palva
The Plant Cell Apr 1995, 7 (4) 391-406; DOI: 10.1105/tpc.7.4.391
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF

In this issue

The Plant Cell
Vol. 7, Issue 4
Apr 1995
  • Table of Contents
  • Index by author

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Cell Preview
  • Archive
  • Teaching Tools in Plant Biology
  • Plant Physiology
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Peer Review Reports
  • Journal Miles
  • Transfer of reviews to Plant Direct
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds
  • Contact Us

Copyright © 2021 by The American Society of Plant Biologists

Powered by HighWire