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Abstract
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An Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase.

T Ueda, N Matsuda, T Anai, H Tsukaya, H Uchimiya, A Nakano
T Ueda
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
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N Matsuda
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
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T Anai
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
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H Tsukaya
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
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H Uchimiya
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
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A Nakano
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
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Published November 1996. DOI: https://doi.org/10.1105/tpc.8.11.2079

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  • Copyright © 1996 by American Society of Plant Biologists

Abstract

The Arabidopsis Ara proteins belong to the Rab/Ypt family of small GTPases, which are implicated in intracellular vesicular traffic. To understand their specific roles in the cell, it is imperative to identify molecules that regulate the GTPase cycle. Such molecules have been found and characterized in animals and yeasts but not in plants. Using a yeast system, we developed a novel method of functional screening to detect interactions between foreign genes and identified this Rab regulator in plants. We found that the expression of the ARA4 gene in yeast ypt mutants causes exaggeration of the mutant phenotype. By introducing an Arabidopsis cDNA library into the ypt1 mutant, we isolated a clone whose coexpression overcame the deleterious effect of ARA4. This gene encodes an Arabidopsis homolog of the Rab GDP dissociation inhibitor (GDI) and was named AtGDI1. The expression of AtGDI1 complemented the yeast sec19-1 (gdi1) mutation. AtGDI1 is expressed almost ubiquitously in Arabidopsis tissues. The method described here indicates the physiological interaction of two plant molecules, Ara4 and GDI, in yeast and should be applicable to other foreign genes.

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An Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase.
T Ueda, N Matsuda, T Anai, H Tsukaya, H Uchimiya, A Nakano
The Plant Cell Nov 1996, 8 (11) 2079-2091; DOI: 10.1105/tpc.8.11.2079

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An Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase.
T Ueda, N Matsuda, T Anai, H Tsukaya, H Uchimiya, A Nakano
The Plant Cell Nov 1996, 8 (11) 2079-2091; DOI: 10.1105/tpc.8.11.2079
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Vol. 8, Issue 11
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