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Differential abscisic acid regulation of guard cell slow anion channels in Arabidopsis wild-type and abi1 and abi2 mutants.

Z M Pei, K Kuchitsu, J M Ward, M Schwarz, J I Schroeder
Z M Pei
Department of Biology, University of California, San Diego, La Jolla 92093-0116, USA.
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K Kuchitsu
Department of Biology, University of California, San Diego, La Jolla 92093-0116, USA.
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J M Ward
Department of Biology, University of California, San Diego, La Jolla 92093-0116, USA.
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M Schwarz
Department of Biology, University of California, San Diego, La Jolla 92093-0116, USA.
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J I Schroeder
Department of Biology, University of California, San Diego, La Jolla 92093-0116, USA.
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Published March 1997. DOI: https://doi.org/10.1105/tpc.9.3.409

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  • Copyright © 1997 by American Society of Plant Biologists

Abstract

Abscisic acid (ABA) regulates vital physiological responses, and a number of events in the ABA signaling cascade remain to be identified. To allow quantitative analysis of genetic signaling mutants, patch-clamp experiments were developed and performed with the previously inaccessible Arabidopsis guard cells from the wild type and ABA-insensitive (abi) mutants. Slow anion channels have been proposed to play a rate-limiting role in ABA-induced stomatal closing. We now directly demonstrate that ABA strongly activates slow anion channels in wild-type guard cells. Furthermore, ABA-induced anion channel activation and stomatal closing were suppressed by protein phosphatase inhibitors. In abi1-1 and abi2-1 mutant guard cells, ABA activation of slow anion channels and ABA-induced stomatal closing were abolished. These impairments in ABA signaling were partially rescued by kinase inhibitors in abi1 but not in abi2 guard cells. These data provide cell biological evidence that the abi2 locus disrupts early ABA signaling, that abi1 and abi2 affect ABA signaling at different steps in the cascade, and that protein kinases act as negative regulators of ABA signaling in Arabidopsis. New models for ABA signaling pathways and roles for abi1, abi2, and protein kinases and phosphatases are discussed.

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Differential abscisic acid regulation of guard cell slow anion channels in Arabidopsis wild-type and abi1 and abi2 mutants.
Z M Pei, K Kuchitsu, J M Ward, M Schwarz, J I Schroeder
The Plant Cell Mar 1997, 9 (3) 409-423; DOI: 10.1105/tpc.9.3.409

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Differential abscisic acid regulation of guard cell slow anion channels in Arabidopsis wild-type and abi1 and abi2 mutants.
Z M Pei, K Kuchitsu, J M Ward, M Schwarz, J I Schroeder
The Plant Cell Mar 1997, 9 (3) 409-423; DOI: 10.1105/tpc.9.3.409
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The Plant Cell
Vol. 9, Issue 3
Mar 1997
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