More articles from BREAKTHROUGH REPORT
Soybean DICER-LIKE2 Regulates Seed Coat Color via Production of Primary 22-Nucleotide Small Interfering RNAs from Long Inverted RepeatsSoybean DCL2 favors long inverted repeat-derived double-stranded RNA as its substrate and generates primary 22-nucleotide siRNAs to regulate seed coat color and silence transposable elements.
Evolutionary Variation in MADS Box Dimerization Affects Floral Development and Protein Abundance in MaizeEvolutionary variants of B-class MADS box transcription factors differentially affect MADS box complex degradation and cause quantitative variation in anther shape in maize.
Vascular Bundles Mediate Systemic Reactive Oxygen Signaling during Light StressThe reactive oxygen species wave propagates through vascular bundles during the systemic response of Arabidopsis (Arabidopsis thaliana) plants to excess light stress.
Establishment of Proximity-Dependent Biotinylation Approaches in Different Plant Model SystemsA systematic study applying a variety of biotin-based proximity labeling approaches in several plant systems using various conditions and bait proteins.
A Fully Functional ROP Fluorescent Fusion Protein Reveals Roles for This GTPase in Subcellular and Tissue-Level PatterningTagging ROP in the middle of the protein produces a functional protein that provides all ROP function in juvenile moss tissues and marks sites with weakened cell walls long before expansion occurs.
Covalent Self-Labeling of Tagged Proteins with Chemical Fluorescent Dyes in BY-2 Cells and Arabidopsis SeedlingsMulticolor imaging with SNAP-tagged tubulin and monitoring of PIN2 endocytosis with a chemical dye demonstrate that specific self-labeling of proteins can be used effectively in plants.
Long-Read cDNA Sequencing Enables a “Gene-Like” Transcript Annotation of Transposable ElementsA transcript-based annotation of transposable elements allows for reduced bioinformatic complexity and direct hypothesis testing of previously challenging regions of the genome.
Identification of Plant Enhancers and Their Constituent Elements by STARR-seq in Tobacco LeavesA high-throughput assay in transiently transformed tobacco leaves identifies enhancers, characterizes their functional elements and detects condition-specific enhancer activity.
The EccDNA Replicon: A Heritable, Extranuclear Vehicle That Enables Gene Amplification and Glyphosate Resistance in Amaranthus palmeriThe eccDNA replicon is a large extranuclear circular DNA that is composed of a sophisticated repetitive structure and harbors the EPSPS and several other genes that are transcribed during glyphosate stress.
Endosidin20 Targets the Cellulose Synthase Catalytic Domain to Inhibit Cellulose BiosynthesisThe cellulose synthase inhibitor Endosidin20 is an excellent tool for manipulating cellulose synthesis in order to produce cellulose products with desired properties and to control weeds.