More articles from BREAKTHROUGH REPORTS
CRISPR-TSKO: A Technique for Efficient Mutagenesis in Specific Cell Types, Tissues, or Organs in ArabidopsisCRISPR-TSKO (tissue-specific knockout) induces somatic mutations and enables the phenotypic analysis of context-specific gene function.
Cryo-EM Structure of Actin Filaments from Zea mays PollenOur cryo-EM structural data, together with the single-molecule magnetic tweezers analysis, reveal that the plant actin filament from Zea mays pollen is more structurally stable than the rabbit skeletal muscle actin filament.
Ribonucleoprotein Capture by in Vivo Expression of a Designer Pentatricopeptide Repeat Protein in ArabidopsisArtificial proteins built from consensus PPR motifs bind the intended RNA in vivo and can be used as RNA affinity tags to purify endogenous RNPs and identify the bound proteins.
Three-Dimensional Time-Lapse Analysis Reveals Multiscale Relationships in Maize Root Systems with Contrasting ArchitecturesTime-lapse 3D imaging, computer vision, and mathematical modeling were used to quantify the root system architectures of three maize genotypes as they grew in complexity from a few to many roots.
Major Domestication-Related Phenotypes in Indica Rice Are Due to Loss of miRNA-Mediated Laccase SilencingAltered lignification mediated by microRNA-directed laccase gene silencing is a major contributor to domestication-associated phenotypes of cultivated indica rice.
Patterned Deposition of Xylan and Lignin is Independent from that of the Secondary Wall Cellulose of Arabidopsis Xylem VesselsA novel cesa7 mutant revealed that patterned deposition of non-cellulosic secondary wall polymers, xylan and lignin, is not dependent on cellulose patterning during xylem vessel differentiation.
A Ycf2-FtsHi Heteromeric AAA-ATPase Complex Is Required for Chloroplast Protein ImportA protease-originated membrane-bound AAA-ATPase is involved in preprotein translocation across the inner envelope membrane of chloroplasts as an essential import motor.
Cellular Ca2+ Signals Generate Defined pH Signatures in PlantsCytosolic Ca2+ transients induced by different stimuli are associated with cytosolic pH transients.
Target of Rapamycin Inhibition in Chlamydomonas reinhardtii Triggers de Novo Amino Acid Synthesis by Enhancing Nitrogen AssimilationTOR inhibition leads to an immediate and substantial accumulation of amino acids, which is driven by an increase in nitrogen assimilation and amino acid synthesis.
Predicting Division Planes of Three-Dimensional Cells by Soap-Film MinimizationA model based on three-dimensional cell shape successfully predicts the division planes of plant and animal cells, confirming a century-old hypothesis.