Skip to main content

Main menu

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • Info for
    • Instructions for Authors
    • Submit a Manuscript
    • Advertisers
    • Librarians
    • Subscribers
  • About
    • Editorial Board and Staff
    • About the Journal
  • More
    • Alerts
    • Contact Us
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Cell
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Cell

Advanced Search

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • Info for
    • Instructions for Authors
    • Submit a Manuscript
    • Advertisers
    • Librarians
    • Subscribers
  • About
    • Editorial Board and Staff
    • About the Journal
  • More
    • Alerts
    • Contact Us
  • Follow PlantCell on Twitter
  • Visit PlantCell on Facebook
  • Visit Plantae
Other
You have accessRestricted Access

Shine-Dalgarno Sequences Play an Essential Role in the Translation of Plastid mRNAs in Tobacco

Lars B Scharff, Miriam Ehrnthaler, Marcin Janowski, Liam H Childs, Claudia Hasse, Jürgen Gremmels, Stephanie Ruf, Reimo Zoschke, Ralph Bock
Lars B Scharff
University of Copenhagen CITY: Thorvaldsensvej 40 Denmark [DK]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Miriam Ehrnthaler
Max Planck Institute of Molecular Plant Physiology CITY: D-14476 Potsdam-Golm Germany [DE]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marcin Janowski
Max Planck Institute of Molecular Plant Physiology CITY: D-14476 Potsdam-Golm Germany [DE]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Liam H Childs
Max Planck Institute of Molecular Plant Physiology CITY: D-14476 Potsdam-Golm Germany [DE]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Claudia Hasse
Max Planck Institute of Molecular Plant Physiology CITY: D-14476 Potsdam-Golm Germany [DE]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jürgen Gremmels
Max Planck Institute of Molecular Plant Physiology CITY: D-14476 Potsdam-Golm Germany [DE]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephanie Ruf
Max Planck Institute of Molecular Plant Physiology CITY: Potsdam POSTAL_CODE: 14476 Germany [DE]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Reimo Zoschke
Max Planck Institute of Molecular Plant Physiology CITY: D-14476 Potsdam-Golm Germany [DE]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ralph Bock
Max Planck Institute of Molecular Plant Physiology CITY: D-14476 Potsdam-Golm POSTAL_CODE: . Germany [DE]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: rbock@mpimp-golm.mpg.de

Published November 2017. DOI: https://doi.org/10.1105/tpc.17.00524

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading
  • © 2017 American Society of Plant Biologists. All rights reserved.

Abstract

In prokaryotic systems, the translation initiation of many, though not all, mRNAs depends on interaction between a sequence element upstream of the start codon (the Shine-Dalgarno sequence, SD) and a complementary sequence in the 3' end of the 16S ribosomal RNA (anti-Shine-Dalgarno sequence, aSD). Although many chloroplast mRNAs harbor putative SDs in their 5' untranslated regions and the aSD displays strong conservation, the functional relevance of SD-aSD interactions in plastid translation is unclear. Here, by generating transplastomic tobacco (Nicotiana tabacum) mutants with point mutations in the aSD coupled with genome-wide analysis of translation by ribosome profiling, we provide a global picture of SD-dependent translation in plastids. We observed a pronounced correlation between weakened predicted SD-aSD interactions and reduced translation efficiency. However, multiple lines of evidence suggest that the strength of the SD-aSD interaction is not the only determinant of the translational output of many plastid mRNAs. Finally, the translation efficiency of mRNAs with strong secondary structures around the start codon is more dependent on the SD-aSD interaction than weakly structured mRNAs. Thus, our data reveal the importance of the aSD in plastid translation initiation, uncover chloroplast genes whose translation is influenced by SD-aSD interactions, and provide insights into determinants of translation efficiency in plastids.

  • Received July 5, 2017.
  • Accepted November 8, 2017.

Log in using your username and password

Forgot your user name or password?

patientACCESS

patientACCESS - Patients desiring access to articles

Pay Per Article - You may access this article (from the computer you are currently using) for 2 days for US$20.00

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

Next
Back to top
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Plant Cell.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Shine-Dalgarno Sequences Play an Essential Role in the Translation of Plastid mRNAs in Tobacco
(Your Name) has sent you a message from Plant Cell
(Your Name) thought you would like to see the Plant Cell web site.
Citation Tools
Shine-Dalgarno interactions in plastids
Lars B Scharff, Miriam Ehrnthaler, Marcin Janowski, Liam H Childs, Claudia Hasse, Jürgen Gremmels, Stephanie Ruf, Reimo Zoschke, Ralph Bock
The Plant Cell Nov 2017, tpc.00524.2017; DOI: 10.1105/tpc.17.00524

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Shine-Dalgarno interactions in plastids
Lars B Scharff, Miriam Ehrnthaler, Marcin Janowski, Liam H Childs, Claudia Hasse, Jürgen Gremmels, Stephanie Ruf, Reimo Zoschke, Ralph Bock
The Plant Cell Nov 2017, tpc.00524.2017; DOI: 10.1105/tpc.17.00524
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF

In this issue

The Plant Cell: 30 (3)
The Plant Cell
Vol. 30, Issue 3
Mar 2018
  • Table of Contents
  • Table of Contents (PDF)
  • Cover (PDF)
  • About the Cover
  • Index by author

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Cell Preview
  • Archive
  • Teaching Tools in Plant Biology
  • Plant Physiology
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Peer Review Reports
  • Journal Miles
  • Transfer of reviews to Plant Direct
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds
  • Contact Us

Copyright © 2018 by The American Society of Plant Biologists

Powered by HighWire