RT Journal Article
SR Electronic
T1 Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region.
JF The Plant Cell
JO Plant Cell
FD American Society of Plant Biologists
SP 641
OP 651
DO 10.1105/tpc.9.4.641
VO 9
IS 4
A1 Anderson, P A
A1 Lawrence, G J
A1 Morrish, B C
A1 Ayliffe, M A
A1 Finnegan, E J
A1 Ellis, J G
YR 1997
UL http://www.plantcell.org/content/9/4/641.abstract
AB The M rust resistance gene from flax was cloned after two separate approaches, an analysis of spontaneous M mutants with an L6 gene-derived DNA probe and tagging with the maize transposon Activator, independently identified the same gene. The gene encodes a protein of the nucleotide binding site leucine-rich repeat class and is related (86% nucleotide identity) to the unlinked L6 rust resistance gene. In contrast to the L locus, which contains a single gene with multiple alleles, approximately 15 related genes occur at the complex M locus, with only one encoding the M resistance specificity. The M protein contains two direct repeats of 147 and 149 amino acids in the C-terminal part of the leucine-rich region. Three mutant alleles of M encoding a product containing a single repeat unit of 154 amino acids were isolated. The mutant DNA sequences probably occurred by unequal intragenic exchange in the coding region of the repeats. The recombinant alleles lost M resistance and gained no detectable new resistance specificity.