PT  - JOURNAL ARTICLE
AU  - Parker, J E
AU  - Coleman, M J
AU  - Szabò, V
AU  - Frost, L N
AU  - Schmidt, R
AU  - van der Biezen, E A
AU  - Moores, T
AU  - Dean, C
AU  - Daniels, M J
AU  - Jones, J D
TI  - The Arabidopsis downy mildew resistance gene RPP5 shares similarity to the toll and interleukin-1 receptors with N and L6.
AID  - 10.1105/tpc.9.6.879
DP  - 1997 Jun 01
TA  - The Plant Cell
PG  - 879--894
VI  - 9
IP  - 6
4099  - http://www.plantcell.org/content/9/6/879.short
4100  - http://www.plantcell.org/content/9/6/879.full
SO  - Plant Cell1997 Jun 01; 9
AB  - Plant disease resistance genes operate at the earliest steps of pathogen perception. The Arabidopsis RPP5 gene specifying resistance to the downy mildew pathogen Peronospora parasitica was positionally cloned. It encodes a protein that possesses a putative nucleotide binding site and leucine-rich repeats, and its product exhibits striking structural similarity to the plant resistance gene products N and L6. Like N and L6, the RPP5 N-terminal domain resembles the cytoplasmic domains of the Drosophila Toll and mammalian interleukin-1 transmembrane receptors. In contrast to N and L6, which produce predicted truncated products by alternative splicing, RPP5 appears to express only a single transcript corresponding to the full-length protein. However, a truncated form structurally similar to those of N and L6 is encoded by one or more other members of the RPP5 gene family that are tightly clustered on chromosome 4. The organization of repeated units within the leucine-rich repeats encoded by the wild-type RPP5 gene and an RPP5 mutant allele provides molecular evidence for the heightened capacity of this domain to evolve novel configurations and potentially new disease resistance specificities.