RT Journal Article
SR Electronic
T1 Light Quality–Dependent Nuclear Import of the Plant Photoreceptors Phytochrome A and B
JF The Plant Cell
JO Plant Cell
FD American Society of Plant Biologists
SP 1445
OP 1456
DO 10.1105/tpc.11.8.1445
VO 11
IS 8
A1 Kircher, Stefan
A1 Kozma-Bognar, Laszlo
A1 Kim, Lana
A1 Adam, Eva
A1 Harter, Klaus
A1 Schäfer, Eberhard
A1 Nagy, Ferenc
YR 1999
UL http://www.plantcell.org/content/11/8/1445.abstract
AB The phytochrome (phy) family of plant photoreceptors controls various aspects of photomorphogenesis. Overexpression of rice phyA–green fluorescent protein (GFP) and tobacco phyB–GFP fusion proteins in tobacco results in functional photoreceptors. phyA–GFP and phyB–GFP are localized in the cytosol of dark-adapted plants. In our experiments, red light treatment led to nuclear translocation of phyA–GFP and phyB–GFP, albeit with different kinetics. Red light–induced nuclear import of phyB–GFP, but not that of phyA–GFP, was inhibited by far-red light. Far-red light alone only induced nuclear translocation of phyA–GFP. These observations indicate that nuclear import of phyA–GFP is controlled by a very low fluence response, whereas translocation of phyB–GFP is regulated by a low fluence response of phytochrome. Thus, light-regulated nucleocytoplasmic partitioning of phyA and phyB is a major step in phytochrome signaling.