RT Journal Article
SR Electronic
T1 Genome-Wide Analysis of Arabidopsis Pentatricopeptide Repeat Proteins Reveals Their Essential Role in Organelle Biogenesis
JF The Plant Cell
JO Plant Cell
FD American Society of Plant Biologists
SP 2089
OP 2103
DO 10.1105/tpc.104.022236
VO 16
IS 8
A1 Lurin, Claire
A1 Andrés, Charles
A1 Aubourg, Sébastien
A1 Bellaoui, Mohammed
A1 Bitton, Frédérique
A1 Bruyère, Clémence
A1 Caboche, Michel
A1 Debast, Cédrig
A1 Gualberto, José
A1 Hoffmann, Beate
A1 Lecharny, Alain
A1 Le Ret, Monique
A1 Martin-Magniette, Marie-Laure
A1 Mireau, Hakim
A1 Peeters, Nemo
A1 Renou, Jean-Pierre
A1 Szurek, Boris
A1 Taconnat, Ludivine
A1 Small, Ian
YR 2004
UL http://www.plantcell.org/content/16/8/2089.abstract
AB The complete sequence of the Arabidopsis thaliana genome revealed thousands of previously unsuspected genes, many of which cannot be ascribed even putative functions. One of the largest and most enigmatic gene families discovered in this way is characterized by tandem arrays of pentatricopeptide repeats (PPRs). We describe a detailed bioinformatic analysis of 441 members of the Arabidopsis PPR family plus genomic and genetic data on the expression (microarray data), localization (green fluorescent protein and red fluorescent protein fusions), and general function (insertion mutants and RNA binding assays) of many family members. The basic picture that arises from these studies is that PPR proteins play constitutive, often essential roles in mitochondria and chloroplasts, probably via binding to organellar transcripts. These results confirm, but massively extend, the very sparse observations previously obtained from detailed characterization of individual mutants in other organisms.