Table 4. Mutation of the PSTVd Variants in a Predicted CalS11-like Targeting vd-sRNA
PSTVd VariantMutated vd-sRNACalS11-like mRNA/Mutant vd-sRNA∆GaMutant
PSTVd-ICUCCUGAGGUCUAAAGAAAAAb3768 GG A GUCUG 3788−13.90PSTVd-Imut
5′-U UUC U CUCAGGAG-3′
3′-A AAG A GAGUCCUC-5′
59 AA A AUCUG 39
PSTVd-ICUCUCGAGCAGAAAAGAAAAAc3768 GG A G AG 3788−18.20PSTVd-I→M
5′-U UUC U UCUGCUC GAG-3′
3′-A AAG A AGACGAG CUC-5′
59 AA A A CU 39
PSTVd-MCUCUCGAGGUCUAAAGAAAAAb3768 GG A GUCUG AG 3788−7.50PSTVd-Mmut
5′-U UUC U CUC GAG-3′
3′-A AAG A GAG CUC-5′
59 AA A AUCUG CU 39
PSTVd-MCUCCUGAGCAGAAAAGAAAAAd3768 GG A G 3788−24.60PSTVd-M→I
5′-U UUC U UCUGCUCAGGAG-3′
3′-A AAG A AGACGAGUCCUC-5′
59 AA A G 39
  • a Hybridization energy in kcal/mol.

  • b The RISC-mediated RNA cleavage site is disrupted.

  • c Produces a PSTVd-M-type sRNA targeting a CalS11-like mRNA.

  • d Produces a PSTVd-I-type sRNA targeting a CalS11-like mRNA.

  • Altered nucleotides are underlined.