Table 1.

Chlorophyll Contents and Chlorophyll Fluorescence Parameters in 4-Week-Old Wild-Type and lqy1 Mutants

Measured ParameterGrowth LightAfter 3-h High Light
Col WTlqy1-1lqy1-2Col WTlqy1-1lqy1-2
Chl a (mg/g FW)1.414 ± 0.0591.413 ± 0.0281.489 ± 0.0691.370 ± 0.0161.385 ± 0.0671.415 ± 0.034
Chl b (mg/g FW)0.490 ± 0.0250.486 ± 0.0090.514 ± 0.0240.491 ± 0.0080.503 ± 0.0240.512 ± 0.012
Chl a/b2.893 ± 0.0292.907 ± 0.0162.894 ± 0.0060.265 ± 0.0242.754 ± 0.0072.764 ± 0.009
Fo0.091 ± 0.0060.139 ± 0.006***0.113 ± 0.005*0.106 ± 0.0060.170 ± 0.006***0.140 ± 0.006**
Fm0.508 ± 0.0280.692 ± 0.023***0.570 ± 0.0180.297 ± 0.0180.334 ± 0.0100.277 ± 0.007
Fv/Fm0.821 ± 0.0050.800 ± 0.005**0.803 ± 0.005*0.644 ± 0.0060.491 ± 0.009***0.497 ± 0.013***
NPQ1.832 ± 0.0111.815 ± 0.0151.835 ± 0.0222.529 ± 0.0382.936 ± 0.050***2.933 ± 0.052***
qE1.388 ± 0.0091.304 ± 0.011***1.365 ± 0.0161.611 ± 0.0401.637 ± 0.0381.661 ± 0.027
qI0.444 ± 0.0070.511 ± 0.010***0.470 ± 0.010*0.918 ± 0.0171.299 ± 0.026***1.272 ± 0.036***
  • Measurements of chlorophyll fluorescence parameters were done on plants after 20 min of dark adaption. For NPQ, qE, and qI measurements, an actinic light treatment (531 μmol photons m−2 s−1) was performed for 715 s. During actinic illumination, 36 saturation pulses (2800 μmol photons m−2 s−1) were applied at 20-s intervals. After termination of actinic light, recovery of Fm was monitored for 14 min as described by Müller et al. (2001). During the recovery, 16 saturation pulses were applied. After a 3-h high-light treatment, NPQ, qE , and qI were calculated with Fm determined before the high-light treatment. Data are presented as means ± se (n = 4 for chlorophyll contents and n = 8 for chlorophyll fluorescence parameters). The asterisk indicates a significant difference between the mutant and Col wild type (WT) (Student’s t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001). FW, fresh weight.