Table 2.

Comparison of the Rate Constant and Half-Time for D1 Turnover and the Relative Rate for D1 Synthesis in Wild-Type and lqy1 Mutants

DegradationSynthesis
GenotypekPI x 103, min−1t1/2, minRelative Synthesis Rate
WT5.87 ± 0.58120 ± 111.00 ± 0.00
lqy1-17.95 ± 1.3694 ± 161.37 ± 0.10
lqy1-27.84 ± 0.7690 ± 91.29 ± 0.05
  • The degradation rate constant (kPI) and degradation half-time (t1/2) for D1 degradation were calculated from data fitted to first order reaction kinetics (A = A0e−kt) according to Sirpiö et al. (2007). Relative rate of D1 synthesis was calculated during a 1-h pulse under high light. Data are presented as means ± se (n = 3). WT, wild type.