Comparison of the Rate Constant and Half-Time for D1 Turnover and the Relative Rate for D1 Synthesis in Wild-Type and lqy1 Mutants
| Degradation | Synthesis | ||
| Genotype | kPI x 103, min−1 | t1/2, min | Relative Synthesis Rate |
| WT | 5.87 ± 0.58 | 120 ± 11 | 1.00 ± 0.00 |
| lqy1-1 | 7.95 ± 1.36 | 94 ± 16 | 1.37 ± 0.10 |
| lqy1-2 | 7.84 ± 0.76 | 90 ± 9 | 1.29 ± 0.05 |
The degradation rate constant (kPI) and degradation half-time (t1/2) for D1 degradation were calculated from data fitted to first order reaction kinetics (A = A0e−kt) according to Sirpiö et al. (2007). Relative rate of D1 synthesis was calculated during a 1-h pulse under high light. Data are presented as means ± se (n = 3). WT, wild type.