Table 2.

Translational Efficiency in Transgenic Rice

ConstructTransgenic Rice LinesRelative mRNA LevelaRelative Luciferase ActivitybTranslation Efficiencyc
LUCWT468-21.001.001.00
468-70.580.831.43
468-80.530.500.94
LUCm123470-20.193.6018.95
470-80.347.2421.29
LUCnew1724-20.557.9314.42
724-50.569.3316.66
724-110.152.8719.13
  • a Relative mRNA levels of transgenes have been normalized to rice actin mRNA level in each sample.

  • b Luciferase activity was measured as light units per microgram of total protein in each sample, with relative luciferase activity of transgenic line 468-2 set as 1.00.

  • c Relative translation efficiency is the relative luciferase activity divided by the relative mRNA level.