Table 7.

Effect of Ca2+ Influx Antagonists EGTA and La3+ on MtENOD12 Induction

ElicitorEffectorConcentrationIncubation Time [Ca2+] (mM) / with Elicitor (hr)% GUS-Staining Plants
NodRma0 or 5 / 395
(10−9 M)a0 or 5 / 6100
EGTA2 mM0 / 3 or 66b
2 mM5 / 6100
2 mM5 (Mg2+)c / 67b
2 mM0/3, followed by 5/394
La3+5 μM0 / 677d
50 μM0 / 611b
100 μM0 / 66b
50 μM1 / 691d
50 μM10 / 6100
50 μM10 (Mg2+)c / 68b
Mastoparana0 / 629b
(10−6 M)EGTA2 mM0 / 60
La3+50 μM0 / 60
  • a Control in the absence of effector.

  • b Very weak response.

  • c Ca2+ replaced by Mg2+.

  • d Clearly reduced response.