Table 1.

Subcellular Distribution of Fluorescent Probes in Barley Aleurone Protoplasts

ProbeLocation of Fluorescence
Glutathione/sulfhydryls
MonochlorobimaneCytoplasm then vacuole
MonobromobimaneCytoplasm then vacuole
Cell Tracker GreenCytoplasm then vacuole
pH
BCECF-AMCytoplasm then vacuole
BCECFNo uptake
Lysosensor yellow/blueVacuole
Calcium
Indo-1 ffCytoplasm
Indo-1 (Bush and Jones, 1987)Cytoplasm
Fluo-3 (Gilroy and Jones, 1992)Cytoplasm
Protease substrate
ZFR-CMACVacuole
Other fluorophores
Lucifer yellowNo uptake
Oregon greenCytoplasm then vacuole
FluoresceinCytoplasm then vacuole