Table 1.

Disease Reaction of Transgenic Plants Transformed with the Xa21 Gene Family Membersa

X. o. oryzae Races
MemberConstructT0 LineR1bR2cR3dR4eR5fR6gK1hK2aiK2bj
A1pB833104-299.5 ± 1.3k10.5 ± 1.29.1 ± 1.411.3 ± 0.910.4 ± 1.111.5 ± 1.3NDlNDND
A2pB843109-1111.5 ± 1.110.2 ± 1.310.4 ± 0.813.5 ± 1.68.5 ± 1.212.7 ± 1.2NDNDND
CpB853110-410.3 ± 0.89.5 ± 1.211.7 ± 0.813.4 ± 1.19.6 ± 1.313.9 ± 1.0NDNDND
Xa21DpB812105-156.0 ± 0.84.8 ± 1.05.4 ± 0.96.2 ± 1.14.2 ± 1.25.3 ± 0.814.5 ± 1.211.6 ± 1.113.4 ± 1.2
Xa21DpB812105-225.5 ± 1.25.8 ± 1.25.6 ± 1.16.5 ± 1.04.8 ± 1.16.3 ± 1.216.4 ± 1.312.2 ± 1.212.5 ± 1.4
Xa21DpB812105-475.3 ± 0.95.4 ± 1.06.0 ± 1.36.7 ± 1.35.0 ± 1.45.8 ± 0.915.5 ± 1.012.9 ± 1.411.6 ± 1.0
Xa21DpB285-9146.4 ± 0.75.6 ± 0.95.4 ± 1.35.8 ± 0.75.0 ± 0.96.6 ± 0.715.0 ± 1.411.3 ± 1.213.3 ± 1.0
Xa21DpB285-9646.2 ± 0.95.8 ± 1.15.9 ± 1.16.5 ± 1.24.9 ± 1.06.3 ± 0.914.5 ± 1.212.6 ± 1.314.4 ± 1.5
Xa21DpB285-9656.4 ± 0.95.7 ± 1.25.8 ± 1.26.7 ± 1.34.7 ± 1.46.1 ± 0.916.5 ± 1.313.6 ± 1.213.8 ± 1.4
EpB37103-2712.0 ± 1.211.5 ± 1.29.7 ± 1.312.0 ± 1.29.8 ± 0.913.2 ± 1.1NDNDND
FpB8065-1610.3 ± 0.811.0 ± 0.710.8 ± 1.114.5 ± 0.911.5 ± 1.213.9 ± 1.0NDNDND
Control
Xa21pC822106-171.0 ± 0.30.5 ± 0.31.1 ± 0.31.9 ± 0.10.75 ± 0.31.5 ± 0.114.5 ± 0.913.8 ± 0.814.7 ± 0.7
TP309None12.0 ± 0.79.5 ± 0.811.0 ± 0.713.7 ± 1.09.4 ± 0.612.5 ± 0.616.4 ± 1.214.0 ± 0.915.3 ± 0.8
  • a The name of family member, construct (diagrammed in Figure 1), and the T0 parental line are indicated. Two-month-old progeny were inoculated with X. o. oryzae races 1 to 6 and three X. o. oryzae Korean isolates representing two races. T1 progeny from two or three independently transformed T0 lines were evaluated for pB833 (Xa21 family member A1), pB843 (Xa21 family member A2), pB853 (Xa21 family member C), pB37 (Xa21 family member E), and pB806 (Xa21 family member F). For simplicity, data from only one independently transformed line are shown because the other two or three lines for these five constructs gave similar results. T1, progeny from the independently transformed T0 lines pB812 and pB285-9 (Xa21 family member Xa21D) are listed separately. T2 progeny from lines 106-17 were used to evaluate Xa21 (Wang et al., 1996). For all segregating lines, the presence of the corresponding transgene was determined by DNA gel blot hybridization with RG103. TP309 is the recipient used in the transformation studies. The lesion length (in centimeters) is the average of at least 18 leaves, and the standard error is shown.

  • b R1, Philippine isolate PX061 (race 1).

  • c R2, Philippine isolate PX086 (race 2).

  • d R3, Philippine isolate PX079 (race 3).

  • e R4, Philippine isolate PX0113 (race 4).

  • f R5, Philippine isolate PX0112 (race 5).

  • g R6, Philippine isolate PX099A (race 6).

  • h K1, Korean isolate DY89031 (race K1).

  • i K2a, Korean isolate CK89021 (race K2).

  • j K2b, Korean isolate JW89011 (race K2).

  • k Average lesion length of T1 or T2 progeny carrying the transgene (cm) ±se.

  • l ND, no data.