Table 1.

Summarya of Nodule Initiation Activity of Natural and Synthetic LCOs on L. corniculatus Transgenic Plants

L. corniculatus Vector ControlL. corniculatus 35S–Le7L. corniculatus Le1
LCOCompositionbWild Type121-1121-3KYlec7lecGRBIlec6BIlec15
ControlCarboxymethylcellulose0 (5)0 (3)0 (2)0 (3)0 (6)0 (4)0 (6)
Fraction 1 of USDA61cNodBe-V (C18:1, MeFuc)9 (13)1 (12)3 (5)4 (6)17 (21)3 (6)5 (7)
Fraction 2 of USDA61NodBe-V (Ac, C18:1, MeFuc)15 (18)2 (23)9 (12)3 (5)19 (22)5 (8)6 (8)
NodBe-IV (C18:1, Fuc, Gro)
Fraction 3 of USDA61NodBe-IV (C18:1, MeFuc)10 (15)2 (19)5 (8)3 (6)15 (24)7 (10)5 (9)
NodBe-IV (Cb, C18:1, MeFuc)
NodBe-IV (Cb, C18:1, Fuc, Gro)
NodBe-IV (Cb, C18:1, NMe, MeFuc)
NodBe-IV (C18:1, NMe, MeFuc)
NodBe-IV (Cb, C18:1, NMe, Fuc, Gro)
LCO of NAD138NodBj-V (C18:1)0 (10)d0 (9)0 (7)0 (5)
LCO-3026LCO-IV (C18:1, Fuc, R-Gro)0 (8)0 (10)0 (8)
LCO-1098eLCO-IV (C16:0)0 (9)0 (8)0 (6)
LCO-2086LCO-IV (C18:1, MeFuc)0 (12)0 (12)0 (7)
LCO-3027LCO-IV (C18:1, Fuc, S-Gro)0 (13)0 (13)0 (6)
  • a The number of sites of cortical cell division after application of 100 ng of test material per spot inoculation. Numbers in parentheses are the total number of roots assayed.

  • b Ac, acetyl; Cb, carbamoyl; Fuc, fucose; Gro, glycerol; Me, methyl; NMe, N-methyl; R-Gro and S-Gro, R-glycerol and S-glycerol; CX:Y, length of fatty acid:number of desaturations.

  • c Fraction 1 of USDA61 is structurally identical to the major fraction produced by B. japonicum USDA110.

  • d Not determined.

  • e After applying LCO-1098, all of the roots stopped growing, so no cortical cell divisions could be detected.