Table 2.

cAMP Rescues the Appressorium Formation Defect in mac1 Mutants of M. griseaa

StrainNo cAMP10 mM cAMP50 mM cAMP
Guy110.7 ± 1.2b36.3 ± 8.090.7 ± 2.5
DA1274.0 ± 1.061.3 ± 9.296.7 ± 2.5
DA1280.7 ± 0.657.3 ± 4.095.7 ± 0.6
DA1291.0 ± 1.069.3 ± 3.191.0 ± 3.6
DA1540.3 ± 0.681.7 ± 6.889.0 ± 3.0
DA1560.7 ± 0.676.7 ± 4.292.0 ± 2.6
  • a Hydrophobic plastic coverslips (Fisher) were used for the macl mutants; pieces of the hydrophilic side of GelBond were used for wild-type strain Guy11.

  • b The percentage of germinated conidia that formed appressoria was quantitated after 24 hr. The mean and standard deviation were calculated using combined data of at least three replicates. One hundred germinated conidia were examined per replicate.