Table 2.

Frequency of DR Plants and Independent Transposition Events in the Progeny of Primary Transformants

TransformantTotal SeedDRFrequencyGUS+aDNA Gel Blot Analysisb% Independent/DR% Independent/Total
8337 No. 116,000281.75 × 10–32414.30.025
8337 No. 24,00012.5 × 10–4011000.025
8337 No. 416,000301.88 × 10–328000
8337 No. 617,60063.4 × 10–44233.30.011
8337 No. 917,60016 × 10–5011000.006
8313 No. 121,600125.5 × 10–40866.70.037
8313 No. 419,60015 × 10–5011000.005
8353 No. 219,20052.6 × 10–42240.00.01
8353 No. 38,00011.2 × 10–40000
8353 No. 412,40021.6 × 10–4NAc21000.016
8353 No. 524,00014 × 10–5NA000
  • a Number of GUS-staining plants.

  • b Number of independent insertions as estimated by DNA gel blot analysis.

  • c NA, not applicable.