Table 1.

Flowering Time of 35S::FLF T1 Transgenic Plants

PlantsaFlowering Time (Days)bRelative Expression Level of FLF mRNAc
C24301
B218>10
B518>10
B11>100>10
B12>100>10
Ler20NDd
B36>100>10
B45>100>10
flf-1>1002
  • a Transgenic T1 plants (in C24: B2, B5, B11, B12; or in Ler: B36, B45) were selected on Murashige and Skoog plates containing 50 mg/L kanamycin and grown at 23°C under fluorescent lights (16 hr of light and 8 hr of dark). Plants were then transferred to soil at 20 days. The other plants were grown under identical conditions but without selection on kanamycin.

  • b Flowering time was recorded as the number of days to stem elongation.

  • c The FLF transcript level was determined in early-flowering T2 progeny for B2 and B5, from young leaves of the T1 late-flowering plants (B11, B12, B36, and B45), and from in vitro–grown C24, Ler, and flf-1 plants.

  • d ND, not detectable.