Table 4.

End Product Analyses of PelC Cleavage of Oligogalacturonates

Oligomer SubstrateaProductsbFrequency (%)Rate (μkat/mg)
(GalpA)3GalpA + 4-en-thrHexpA-GalpA1000.17
(GalpA)4GalpA + 4-en-thrHexpA-(GalpA)2253 to 4
(GalpA)2 + 4-en-thrHexpA-GalpA75
(GalpA)5(GalpA)2 + 4-en-thrHexpA-(GalpA)27110.1
(GalpA)3 + 4-en-thrHexpA-GalpA29
(GalpA)6(GalpA)2 + 4-en-thrHexpA-(GalpA)3916.1
(GalpA)3 + 4-en-thrHexpA-(GalpA)253
(GalpA)4 + 4-en-thrHexpA-GalpA38
(GalpA)7(GalpA)2 + 4-en-thrHexpA-(GalpA)4422.7
(GalpA)3 + 4-en-thrHexpA-(GalpA)39
(GalpA)4 + 4-en-thrHexpA-(GalpA)255
(GalpA)5 + 4-en-thrHexpA-GalpA32
L-Gal-onic-(GalpA)2No cleavage0
L-Gal-onic-(GalpA)3GalpA + 4-en-thrHexpA-GalpA-L-Gal-onic1000.1
L-Gal-onic-(GalpA)4(GalpA)2 + 4-en-thrHexpA-GalpA-L-Gal-onic321.2
GalpA + 4-en-thrHexpA-(GalpA)2-L-Gal-onic68
L-Gal-onic-(GalpA)5(GalpA)4 + 4-en-thrHexpA-L-Gal-onic24.0
(GalpA)3 + 4-en-thrHexpA-GalpA-L-Gal-onic16
(GalpA)2 + 4-en-thrHexpA-(GalpA)2-L-Gal-onic82
L-Gal-onic-(GalpA)6(GalpA)5 + 4-en-thrHexpA-L-Gal-onic84.0
(GalpA)4 + 4-en-thrHexpA-GalpA-L-Gal-onic21
(GalpA)3 + 4-en-thrHexpA-(GalpA)2-L-Gal-onic66
(GalpA)2 + 4-en-thrHexpA-(GalpA)3-L-Gal-onic5
  • a L-Gal-onic refers to L-galactonic acid or the reduced form of galacturonic acid.

  • b 4-En-thrHexpA refers to a-L-eno-threohexosylpyranosyluronic acid or the 4,5-unstaurated form of galacturonic acid.