Table 1.

Effects of CHX Concentration on Protein Synthesis in Gametophytes

CHX (μM)aCPMbIncorporation (%)c
010,166100
55,70056
103,40834
202,95129
  • a CHX and 35S-L-methionine were added to gametophytes at 3 hr and 20 min and at 3 hr, respectively, before harvesting.

  • b Total protein was extracted from each gametophyte population and trichloroacetic acid precipitated, and the tricholoracetic acid precipitate was counted.

  • c Percentage of 35S-L-methionine incorporated into total protein relative to no CHX treatment control.