Table 2.

Effects of Varying the Time of CHX Treatment on Protein Synthesis in Gametophytes

Time (hr) in CHXaCPMbIncorporation (%)c
087,002100
0.512,84015
1.07,5859
2.06,4257
3.07,6359
4.06,5558
  • a Gametophytes were incubated for various periods of time in 20 μM CHX before adding 35S-L-methionine; gametophytes were then cultured for 3 hr before harvesting.

  • b Total protein was extracted from each gametophyte population and trichloroacetic acid precipitated, and the tricholoracetic acid precipitate was counted.

  • c Percentage of 35S-L-methionine incorporated into total protein relative to no CHX treatment control.