Table 1.

Purification of Plasma Membranes from Lily Pollen Protoplasts^{^a}

Vesicles	Plasma Membrane^{^b}	Vacuolar Membrane^{^c}	Mitochondrial Membrane^{^d}	ER Membrane^{^e}	Golgi Membrane^{^f}
U₃^{^g}	152.10 ± 17.00	6.60 ± 0.21	13.19 ± 0.11	0.04 ± 0.01	4.38 ± 2.36
U₃^{^h}	57.10 ± 18.60	54.90 ± 18.31	286.86 ± 87.81	12.56 ± 1.14	38.88 ± 1.74
LF^ⁱ	14.09 ± 1.88	14.50 ± 3.62	153.74 ± 45.22	15.18 ± 0.21	658.75 ± 28.11

↵^a Enzyme activity is given in nanomoles of Pi per minute per milligram of protein or nanomoles of NADP+ per minute per milligram of protein.
↵^b The plasma membrane marker is VO₄⁻³ ATPase.
↵^c The vacuolar membrane marker is NO₃⁻ ATPase.
↵^d The mitochondrial membrane marker is N₃⁻ ATPase.
↵^e The endoplasmic reticulum (ER) marker is cytochrome c reductase (at pH 7.5). The marker is antimycin A–insensitive NADPH:cytochrome c reductase.
↵^f The Golgi membrane marker is UDPase.
↵^g Vesicles from the third upper fraction.
↵^h Vesicles from the third upper fraction of the first lower fraction.
↵ⁱ Vesicles from the third lower fraction of the first lower fraction.