Table 1.

Purification of Plasma Membranes from Lily Pollen Protoplastsa

VesiclesPlasma MembranebVacuolar MembranecMitochondrial MembranedER MembraneeGolgi Membranef
U3g152.10 ± 17.006.60 ± 0.2113.19 ± 0.110.04 ± 0.014.38 ± 2.36
U3h57.10 ± 18.6054.90 ± 18.31286.86 ± 87.8112.56 ± 1.1438.88 ± 1.74
LFi14.09 ± 1.8814.50 ± 3.62153.74 ± 45.2215.18 ± 0.21658.75 ± 28.11
  • a Enzyme activity is given in nanomoles of Pi per minute per milligram of protein or nanomoles of NADP+ per minute per milligram of protein.

  • b The plasma membrane marker is VO4−3 ATPase.

  • c The vacuolar membrane marker is NO3 ATPase.

  • d The mitochondrial membrane marker is N3 ATPase.

  • e The endoplasmic reticulum (ER) marker is cytochrome c reductase (at pH 7.5). The marker is antimycin A–insensitive NADPH:cytochrome c reductase.

  • f The Golgi membrane marker is UDPase.

  • g Vesicles from the third upper fraction.

  • h Vesicles from the third upper fraction of the first lower fraction.

  • i Vesicles from the third lower fraction of the first lower fraction.