Table 1.

Effects of PAL Overexpression on Phenolic Compounds in Tobacco Cell Suspension Cultures

Unlabeled PrecursorPlant Lineat-CAb4-CAbCafAbFerAbpHBAbScopbSAbVAbVan-Db
NoneWT59310342109211015919
NoneOX121095256818573182209
10–4 M t-CAWT60227136641812313716490
10–4 M t-CAOX17061820121918478139263426
  • a Soluble phenolic compounds were isolated from wild-type (WT) and PAL-overexpressing (OX) tobacco cell suspension cultures 24 hr after feeding with 3H-l-Phe (10–4 M) in the presence or absence of unlabeled trans-cinnamic acid (t-CA).

  • b Amounts of phenolic compounds, quantified after enzymatic hydrolysis, are given as nanomoles per gram fresh weight. The compounds are trans-cinnamic acid, 4-coumaric acid (4-CA), caffeic acid (CafA), ferulic acid (FerA), pHBA, scopoletin (Scop), salicylic acid (SA), vanillic acid (VA), and vanillin derivative (Van-D). The experiments were conducted as described in the legend to Figure 3, and a duplicate experiment gave essentially the same results.