Table 1.

Complementation of Fertility and Stem Cuticle Phenotype of cer6-2 Mutants with CER6

LineaStem Epicuticular WaxbSeeds (n)cKan Resistanceb,dWT CER6b,e
Ler+++27.2 ± 2.9 (23)NA+
cer6-2 0.3 ± 0.6 (14)NA
6-2 FJ+++29.5 ± 2.0 (12)++
6-2 FG+++27.4 ± 4.7 (15)++
6-2 FI+++24.4 ± 6.0 (18)++
6-2 R1+++22.2 ± 2.9 (15)++
6-2 FF+++20.8 ± 2.7 (18)++
6-2 RA++28.3 ± 3.3 (19)++
6-2 F4++21.6 ± 4.1 (19)++
6-2 FK+21.8 ± 5.1 (8)++
6-2 F5+11.5 ± 3.0 (13)++
6-2 FH– /+26.1 ± 3.3 (19)++
6-2 F3– /+23.1 ± 3.1 (16)++
6-2 RB26.3 ± 2.2 (16)++
6-2 FD15.9 ± 3.3 (14)++
6-2 FA0.4 ± 1.0 (10)+
6-2 FB0.4 ± 0.7 (10)+
  • a Lines 6-2 FJ through 6-2 FD denote cer6-2 lines transgenic for a wild-type copy of CER6, ranked by their stem cuticle phenotypes. Lines 6-2 FA and 6-2 FB contain vector sequences but lack CER6. Ler, Landsberg erecta.

  • b (+ + +), wild-type wax levels; (++), (+), (–/+), intermediate wax; (–), no wax.

  • c Mature seeds per half seed pod and standard deviation.

  • d The presence of the kanamycin resistance gene was determined by PCR. NA, not applicable.

  • e The presence of wild-type (WT) CER6 was assessed using two dCAPS markers.