Table 1.

Complementation of Fertility and Stem Cuticle Phenotype of cer6-2 Mutants with CER6

Line^{^a}	Stem Epicuticular Wax^{^b}	Seeds (n)^{^c}	Kan Resistance^{^b,^d}	WT CER6^{^b,^e}
Ler	+++	27.2 ± 2.9 (23)	NA	+
cer6-2	–	0.3 ± 0.6 (14)	NA	–
6-2 FJ	+++	29.5 ± 2.0 (12)	+	+
6-2 FG	+++	27.4 ± 4.7 (15)	+	+
6-2 FI	+++	24.4 ± 6.0 (18)	+	+
6-2 R1	+++	22.2 ± 2.9 (15)	+	+
6-2 FF	+++	20.8 ± 2.7 (18)	+	+
6-2 RA	++	28.3 ± 3.3 (19)	+	+
6-2 F4	++	21.6 ± 4.1 (19)	+	+
6-2 FK	+	21.8 ± 5.1 (8)	+	+
6-2 F5	+	11.5 ± 3.0 (13)	+	+
6-2 FH	– /+	26.1 ± 3.3 (19)	+	+
6-2 F3	– /+	23.1 ± 3.1 (16)	+	+
6-2 RB	–	26.3 ± 2.2 (16)	+	+
6-2 FD	–	15.9 ± 3.3 (14)	+	+
6-2 FA	–	0.4 ± 1.0 (10)	+	–
6-2 FB	–	0.4 ± 0.7 (10)	+	–

↵a Lines 6-2 FJ through 6-2 FD denote cer6-2 lines transgenic for a wild-type copy of CER6, ranked by their stem cuticle phenotypes. Lines 6-2 FA and 6-2 FB contain vector sequences but lack CER6. Ler, Landsberg erecta.
↵b (+ + +), wild-type wax levels; (++), (+), (–/+), intermediate wax; (–), no wax.
↵c Mature seeds per half seed pod and standard deviation.
↵d The presence of the kanamycin resistance gene was determined by PCR. NA, not applicable.
↵e The presence of wild-type (WT) CER6 was assessed using two dCAPS markers.