Complementation of Fertility and Stem Cuticle Phenotype of cer6-2 Mutants with CER6
Linea | Stem Epicuticular Waxb | Seeds (n)c | Kan Resistanceb,d | WT CER6b,e |
---|---|---|---|---|
Ler | +++ | 27.2 ± 2.9 (23) | NA | + |
cer6-2 | – | 0.3 ± 0.6 (14) | NA | – |
6-2 FJ | +++ | 29.5 ± 2.0 (12) | + | + |
6-2 FG | +++ | 27.4 ± 4.7 (15) | + | + |
6-2 FI | +++ | 24.4 ± 6.0 (18) | + | + |
6-2 R1 | +++ | 22.2 ± 2.9 (15) | + | + |
6-2 FF | +++ | 20.8 ± 2.7 (18) | + | + |
6-2 RA | ++ | 28.3 ± 3.3 (19) | + | + |
6-2 F4 | ++ | 21.6 ± 4.1 (19) | + | + |
6-2 FK | + | 21.8 ± 5.1 (8) | + | + |
6-2 F5 | + | 11.5 ± 3.0 (13) | + | + |
6-2 FH | – /+ | 26.1 ± 3.3 (19) | + | + |
6-2 F3 | – /+ | 23.1 ± 3.1 (16) | + | + |
6-2 RB | – | 26.3 ± 2.2 (16) | + | + |
6-2 FD | – | 15.9 ± 3.3 (14) | + | + |
6-2 FA | – | 0.4 ± 1.0 (10) | + | – |
6-2 FB | – | 0.4 ± 0.7 (10) | + | – |
↵a Lines 6-2 FJ through 6-2 FD denote cer6-2 lines transgenic for a wild-type copy of CER6, ranked by their stem cuticle phenotypes. Lines 6-2 FA and 6-2 FB contain vector sequences but lack CER6. Ler, Landsberg erecta.
↵b (+ + +), wild-type wax levels; (++), (+), (–/+), intermediate wax; (–), no wax.
↵c Mature seeds per half seed pod and standard deviation.
↵d The presence of the kanamycin resistance gene was determined by PCR. NA, not applicable.
↵e The presence of wild-type (WT) CER6 was assessed using two dCAPS markers.