Table 1.

iar1 Mutant Alleles

AlleleAccessionScreenMutagenCodon ChangeaAmino Acid ChangeAllele Detection
iar1-1WsIAA-AlaREMSGGA→AGAGly400→ArgDestroys HpaII site
iar1-2WsIAA-AlaREMSGGA→AGAGly423→ArgDestroys AciI site
iar1-3Col-0IAA-AlaRFast neutronGGAATG→GGGCCTGFrameshift at codon 458Creates HaeIII site
iar1-4Col-0IAA-AlaRFast neutron65 to 80-kb deletion including entire IAR1 coding regionLate-flowering due to FKF1 deletionb
iar1-5Wsilr1 enhancer IAA-LeuREMSAGA→AAAArg357→LysSequencing
iar1-6Wsilr1 enhancer IAA-LeuREMSCAA→TAAGln162→stopDestroys HphI site
iar1-7Wsilr1 enhancer IAA-PheREMStagCT→taaCT (3′ splice site of first intron)cPredicted to disrupt splicingCreates AluI site
  • a Nucleotide changes are underlined.

  • b Nelson et al., 2000.

  • c Intron bases are in lowercase letters; exon bases are in uppercase letters.