Table 2.

NBD-PS Transport in Microsomal Vesicles Reconstituted in the Presence of Solubilized Membrane Proteins as Indicated

Controls–ATPa,b(%) 1ATPa,c (%)
Yeast DRS2 microsomes48 ± 529 ± 4
Yeast drs2 microsomes51 ± 342 ± 6
Yeast DRS2 microsomes +
vanadate (200 μM)
46 ± 445 ± 3
No added protein49 ± 351 ± 3
Plasma membrane H+-ATPase47 ± 346 ± 2
  • a a Values are given as inner leaflet NBD-PS (%) and are the means 6SD of three measurements from three independent reconstitution experiments. Protein-to-lipid ratios were 1:15.

  • b ATP was omitted from the assay.

  • c ATP (3 mM) was added to start the reaction.