Inhibition of Leaf Primordium Formation and Induction of Ectopic Leaf Primordia by Local Application to Tomato Apices of Lanolin Paste Containing Various Auxin Transport Inhibitors or Auxin
Treatmenta | No. of Treatments | Site of Application | No. of Effects (%) |
---|---|---|---|
Lanolin only | 22 | I1 | 0 |
Lanolin only | 15 | I2 | 0 |
10 mM NPA | 22 | I1 | 17b (77) |
10 mM HFCA | 12 | I1 | 8b (66) |
10 mM TIBA | 12 | I1 | 5b (42) |
10 mM apigenin | 12 | I1 | 0 |
10 mM quercetin | 12 | I1 | 0 |
10 mM IAA | 14 | I1 | 10c (71) |
10 mM IAA | 64 | I2 | 22d (34) |
10 mM IAA | 14 | Entire | 7e (50) |
↵a All treatments were performed by applying lanolin paste containing the indicated substances to freshly dissected tomato apices at the flank of the meristems either at the position of incipient leaf formation (I1) or at the site where the following primordium was expected (I2). Alternatively, the entire flank was treated. Apices were examined 7 days after treatment.
↵b Leaf formation inhibited.
↵c Primordia thicker than normal.
↵d Ectopic primordia fused to P1.
↵e Large ectopic primordia fused to P1.