Table 1.

Relative Transcript Abundance after Wounding, Dehydration, or Insect (Pieris rapae) Feedinga

WoundingDehydrationP. rapae
GeneDescriptionbWild Typecoi1-1Wild TypeWild Type
COI1-dependent genesc
    ACO1Aminocyclopropane–carboxylic acid oxidase  2.2  1.8  1.21.5
    ASA1Anthranilate synthase (α subunit)  3.1  1.0  2.71.7
    ASBAnthranilate synthase (β subunit)  2.3  1.2  2.51.7
    AOSAllene oxide synthase  4.8  0.6  4.82.1
    AWI31Unknown  2.7  1.7  2.21.8
    CCRCinnamoyl–coA reductase  2.1  1.2  2.41.3
    CHSChalcone synthase  2.0  1.0  3.02.1
    4CL4-Coumarate:coA ligase  2.8  1.5  2.01.3
    COMTO-methyltransferase  4.7  1.6  3.61.4
    CYP83B1Cytochrome P450  2.9  1.0  4.41.8
    FAD7Fatty acid desaturase  4.7  1.2  2.71.5
    HELHevein-like protein  1.1  1.2  0.72.8
    HPLHydroperoxide lyase  4.8  1.0  7.12.2
    JIPJasmonate-inducible protein  2.9  1.6  2.53.1
    JR3Aminohydrolase  9.3  1.9  8.93.6
    LOX2Lipoxygenase  3.2  0.7  4.92.3
    MBPMyrosinase binding protein  2.6  1.0  2.22.1
    MPK3Mitogen-activated protein kinase  0.9  2.2  1.11.5
    MT1Metallothionein  2.2  1.5  1.92.1
    NPR1Transcription factor inhibitor  1.2  2.4  0.91.0
    PAL2Phenylalanine ammonia-lyase  5.6  1.4  1.10.3
    PR-2β-1-3-Glucanase  3.0  1.5  3.81.6
    SAHHS-adenosyl-l-homocysteine hydrolase  2.2  1.4  1.21.0
    TSATryptophan synthase (α subunit)  6.3  1.0  3.72.0
    TSBTryptophan synthase (β subunit)  2.7  1.3  2.61.8
COI1-independent genesc
    ACX1Acyl-coA oxidase  8.3  2.412.61.7
    CM1Chorismate mutase  2.1  2.1  2.61.7
    DBPOligogalacturonide binding protein homolog  2.7  2.6  2.31.4
    ER5Late embryogenesis abundant-like protein  6.613.811.22.4
    ERF4Ethylene-responding factor  4.0  3.1  4.62.2
    GPX2Glutathione peroxidase  2.0  2.3  4.91.7
    GST1Glutathione S-transferase  9.212.0  2.22.1
    GST5Glutathione S-transferase  6.2  2.711.52.9
    OECOxygen-evolving protein  3.0  2.5  2.11.2
    OPR1OPDA reductase  9.3  7.7  1.51.7
    PAL1Phenylalanine ammonia-lyase  3.8  2.1  3.51.6
    PGIPPolygalacturonase-inhibiting protein  2.9  7.3  4.51.8
    PME1Pectin methyl esterase  2.0  2.2  0.71.1
    PR3AIVChitinase  2.9  7.6  0.71.8
    PRODHProline dehydrogenase11.610.4  0.032.4
    TCH1Calmodulin  2.4  5.2  1.41.6
    TCH2Calmodulin-related protein  2.4  4.5  0.81.3
    TCH3Calmodulin-related protein  2.4  4.6  0.31.3
    TCH4Endotransglycosylase  3.7  3.7  1.01.9
    RNS1RNase64.652.422.12.3
    XERO2Dehydrin-like protein23.883.413.93.1
  • a Samples from wounded (90 min), dehydrated (120 min), or insect-challenged (180 min) Arabidopsis leaves were fluorescently labeled with Cy5-dCTP, and respective control samples (untreated) were labeled with Cy3-dCTP. After hybridization with a cDNA microarray and scanning, expression ratios were calculated. Ratios correspond to fluorescent values from treated plants relative to untreated plants.

  • b For further details, see http://www.unil.ch/ibpv.

  • c Genes induced (having a ratio >2.0) in wild-type plants after wounding as well as the P. rapae–inducible HEL and two genes (MPK3, NPR1) only induced in the wounded coi1-1 mutant are included. Genes that are induced after wounding in both wild-type and coi1-1 plants are considered independent of the COI1 pathway. Our classification of COI1-dependent or -independent genes simplifies data analysis; we do not imply that this simplification exists in nature.