Table 3.

Known Nitrate-Responsive Genes: Microarray and RNA Gel Blot Data

MicroarraybRNA Gel Blotb
GeneaL-20minH-2hrL-20minL-2hrH-20minH-2hr
Nitrate uptake and assimilation
    NR (NIA1)  5.5 (1.0)  6.3 (1.6)    5.6  2.3  12    9.6
    NR (NIA2)  3.5 (0.2)  4.9 (0.5)    8.9  2.4  16  31
    NiR11 (5.4)16 (4.3)14040130130
    UPM111 (3.8)13 (2.4)  13  2.2  17  17
    NRT1;1 (CHL1)c  2.6 (0.5)  1.6 (0.0)    4.4  3.4    2.8    2.7
    NRT2NANA    2.0  2.1    2.3  18
Fd reduction
    Fd  3.6 (1.0)  4.5 (0.5)    5.2  3.2    3.9    3.9
    FNR chromosome IV  6.2 (1.2)  8.3 (0.1)    6.4  7.6  17  17
    FNR chromosome I  5.5 (1.6)  9.0 (1.1)  5636  43  69
Oxidative pentose phosphate pathway
    G6PDH (clone E5)  5.1 (1.7)  4.4 (0.8)  20  4.0  35  36
    G6PDH (chromosome I)  6.4 (3.1)  6.3 (1.6)  18  3.6  12    9.5
    6PGDH  6.8 (2.4)  7.1 (0.8)NDNDNDND
Ammonium assimilation
    Glutamine synthetase  1.2 (0.1)  2.5 (0.1)NDNDNDND
    Fd-GOGATNSNSNDNDNDND
    NADH-GOGATc  1.7 (0.1)  2.8 (0.5)NDNDNDND
Organic acid metabolism
    PEP carboxylasec  1.3 (0.1)  1.1 (0.1)NDNDNDND
  • a Gene symbols: NRT1;1 (CHL1), dual-affinity nitrate transporter; NRT2, high-affinity nitrate transporter; G6PDH, glucose-6-P-dehydrogenase; 6PGDH, 6-phosphogluconate dehydrogenase. Chr., chromosome.

  • b L refers to inductions with 250 μM. H refers to inductions with 10 mM nitrate for the arrays and 5 mM for the RNA gel blots. Values for the arrays are averages for all clones and hybridizations for each gene. Numbers in parentheses are standard deviations. NA, not on microarray; ND, not determined; NS, on array but no signal.

  • c Indicates more than one clone was present on the array so that several values were averaged to give the number in the table.