Table 4.

New Nitrate-Responsive Genes: Microarray and RNA Gel Blot Data

MicroarraybRNA Gel Blotb
GeneaL- 20minH-2hrL-20minL-2hrH-20minH-2hr
Nonoxidative pentose pathway
    Transaldolasec  2.2 (0.1)  4.8 (0.1)  5.1  6.3  2.214
    Transketolase  1.6 (0.1)  2.8 (0.2)  2.6  4.3  2.6  4.3
Calcium transport
    Ca-antiporter/CAX1c  2.4 (0.1)  2.3 (0.1)  5.4  2.1  5.312
Transcription factors
    Myb protein  1.8 (0.1)  2.4 (0.3)  2.0  1.7  4.3  4.0
    LHY-myb protein–1.1–1.1  1.8  2.8–2.0  1.6
Protein phosphorylation
    Putative S/T PKase  1.6 (0.0)  4.2 (0.6)  3.6  1.9  2.7  5.0
    NPK1-like PKase  2.3 (0.9)  1.3 (0.1)  4.9–1.720  4.2
Additional genes
    SAG21  3.1 (0.1)  4.5 (0.2)12  4.0  4.1  5.2
    Auxin-induced gene  2.4 (0.9)  3.7 (1.2)  7.2  2.513  8.1
    Sugar-transporter  2.4 (0.1)  1.7 (0.1)  5.1  1.7  3.6–1.3
    His decarboxylase  2.3 (0.3)  2.7 (0.3)  4.2  1.5  3.3  3.6
    Malate dehydrogenase  2.2d  1.8 (0.1)  3.9  2.2  2.8  2.4
    Hemoglobin/AHB1  1.8 (0.1)  5.8 (1.5)  3.2  3.2  2.6  4.7
    Asn synthetase 2  1.3 (0.2)  4.8 (1.5)  1.9  1.71733
    Methyltransferase 1  1.2 (0.1)  4.2 (0.1)  1.9  5.4  1.4  5.6
  • a Gene symbols: CAX1, high-capacity calcium antiporter; LHY, late elongated hypocotyl; SAG21, senescence-associated protein; S/T Pkase, serine/threonine protein kinase; AHB1, class 1 nonsymbiotic hemoglobin.

  • b L refers to inductions at 250 μM nitrate. H refers to inductions at 10 mM nitrate for the arrays and 5 mM for the RNA gel blots. Numbers in parentheses are standard deviations.

  • c Indicates more than one clone was present on the array so that several values were averaged to give the number in the table.

  • d This ratio was based on a single measurement.