Table 1.

Wild-Type and Mutant Proteins Used in This Study a

Aux/IAA ProteinsMutationsDomain IDomain IIDomain III
IAA3Wild type (wt)ETELRLGLPGGWPPVRSYPYLRKIDL
mIIbGWSPVRSY
IAA7Wild type (wt)ATELCLGLPGGWPPVRNYPYLRKVDL
mIaATELCFGLPG
mIbATVRCLGLPG
mIIGWSPVRNY
mIa/mIIATELCFGLPGGWSPVRNY
mIb/mIIATVRCLGLPGGWSPVRNY
mIIIPYLKKVDL
mII/mIIIGWSPVRNYPYLKKVDL
IAA17Wild type (wt)ETELCLGLPGGWPPVRSYPYLRKIDL
mIETVRCLGLPG
mIIGWPLVRSY
mI/mIIETVRCLGLPGGWPLVRSY
mIIIPYLRKIES
IAA19Wild type (wt)ITELRLGLPGGWPPVCSYDKLFGFRGI
dIDeletion of box I (1 to 34 amino acids)
mIIGWSPVCSY
dI/mIIDeletion of box I (1 to 34 amino acids)GWSPVCSY
mII/mIIIGWSPVCSYDKESGFRGI
  • a The amino acids mutated within a conserved region of each domain are indicated in boldface.

  • b No change from wild type.