Table 1.

Binding of Recombinant Peroxidases Present in Sf9 Cell Culture Medium to Ca2+-Pectate

Culture MediumaPelletb
Transfection VectorΔA470 nm min–1Protein (μg)Specific ActivitycΔA470 nm min–1 (% Medium)dProtein (μg)Specific Activity
pVLAPRX13.4131110.215.6 (116)21.5725
pVLM116.41677$#x00A0;$#x00A0;9.8$#x00A0;$#x00A0;7.1 (43)30.2235
pVLM218.8188210.0$#x00A0;$#x00A0;3.8 (20)47.2$#x00A0;$#x00A0;80.5
pVLM313.41684$#x00A0;$#x00A0;7.9$#x00A0;$#x00A0;0.18 (1)38.4$#x00A0;$#x00A0;4.7
  • a Peroxidase activity (ΔA470 nm min–1) and protein amount in 1 mL of culture medium.

  • b Peroxidase activity (ΔA470 nm min–1) and protein content of pellets obtained by centrifugation of 1 mL of culture medium after the addition of 10 μg of PGA and 2 mM CaCl2.

  • c ΔA470 nm min–1 mg protein–1.

  • d Percentage of activity initially present in the medium recovered in the pellet.