Table 1.

Levels of Expression of α-Amylase and GAMyb in the Wild Type and the slender Mutant Barleya

Expression of α-Amylase–GUSExpression of GAMyb–GUS
Hormone TreatmentWild Typeslender MutantWild Typeslender Mutant
No hormone$#x00A0;$#x00A0;0.2 ± 0.0$#x00A0;$#x00A0;8.7 ± 1.214.4 ± 2.020.5 ± 2.1
+GA316.6 ± 2.710.3 ± 1.222.9 ± 1.322.2 ± 3.8
  • a Either the α-amylase or the GAMyb reporter construct was bombarded into slender mutant embryoless half-seed along with the internal control construct UBI–luciferase, using the same amount of effector and reporter constructs (1.14 μg per shot). Transformed half-seeds were incubated for 12 hr with or without 1 μM GA3. GUS activities (× 10–3) ±se are presented.